Spheroid culture of primary hepatocytes with short fibers as a predictable in vitro model for drug screening.
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Spheroid culture of primary hepatocytes with short fibers as a predictable in vitro model for drug screening.
Establishment of a reliable in vitro liver models for drug screening remnants challenge with respect to the withdrawal of hepatocyte spheroids growth and adapt to the current high-throughput systems. In this study, short fibers used as a scaffold for the generation of controlled size hepatocyte spheroids that recapitulate in vivo phenotype and function of the heart. Spheroid formation is modulated by the length and galactose / RGD graft of short fibers, and fibers of short length 50 m motivating the formation of spherical with optimal liver function. short fibers distributed throughout the entire round for the withdrawal of hepatocyte growth compact to form spheroids.
Compared to free scaffolding spheroid culture in agarose-coated plates, culture round with short fibers achieve higher clearance level of the drug model and provide a better prediction of in vivo drug clearance rate with 0.886 correlation value.
In addition, the ability of drug metabolism is very sensitive to metabolic enzyme inducers and inhibitors, and responsiveness was maintained for 20 days of culture, showed efficient in vitro models to determine drug-drug interactions. Therefore, cultural round with short fibers provide strategies easily manipulated to maintain hepatocyte function for a long period and allowing ready to be placed in a conventional multiwell plates and a variety of tools organ-on-a-chip for high-throughput drug screening.
Spheroid culture of primary hepatocytes with short fibers as a predictable in vitro model for drug screening.
3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improved Human hepatocytes (C3A) Function and Polarity.
3D in vitro hepatocyte culture is a core aspect of liver tissue engineering. However, the conventional 3D culture can not maintain hepatocyte polarity, functional phenotype, or survival. Here, we employ a microfluidic chip technology combined with naturally alginate hydrogel to build a 3D liver tissue mimicking liver dish.
We comprehensively evaluated the survival of cultured hepatocytes, functions, and polarity. transcriptome sequencing was used to analyze changes in hepatocyte polarity pathway. The data show that, with increasing duration of the culture, the viability, function, polarity, mRNA expression, and ultrastructure of liver hepatocytes plate mimetic enhanced 3D.
Furthermore, liver dishes 3D mimetic culture can promote changes in bile secretion pathway through effector mechanisms associated with nuclear receptors, bile uptake and efflux transporters. This study provides a scientific basis and evidence for the physiological structure of the bionic hearts prepared using 3D culture.
Liver Dissociation System 2 (Hepatocytes, Parenchymal hepatocytes), Mouse and Rat
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Rat Membrane primary amine oxidase (AOC3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Rat Membrane primary amine oxidase(AOC3) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Rat Membrane primary amine oxidase(AOC3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Membrane primary amine oxidase in samples from serum, plasma, tissue homogenates and other biological fluids.
Rat Aoc3(Membrane primary amine oxidase) ELISA Kit
Description: This product includes 0.5 ml of 10 x PPA dye, 5.5 ml of Assay buffer, 5 ml of reagent A and 0.05 ml of 1 mM methylamine-HCl. It is for measurement of 100 samples using 96-well plates. Cuvettes may also be used for measurements.
Western blot Kit for Rat Primary Antibodies, Chemilum. Substrate
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Breast PrimaCell: Normal Mammary Epithelial Primary Cells Growth Medium
Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Human Membrane primary amine oxidase (AOC3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Human Membrane primary amine oxidase(AOC3) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Human Membrane primary amine oxidase(AOC3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Mouse Membrane primary amine oxidase (AOC3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse Membrane primary amine oxidase(AOC3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Human Myeloid differentiation primary response protein MyD88 (MYD88)
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Membrane primary amine oxidase in samples from serum, plasma, tissue homogenates and other biological fluids.
ELISA kit for Human Membrane primary amine oxidase
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Membrane primary amine oxidase in samples from serum, plasma, tissue homogenates and other biological fluids.
Mammary Dissociation System 5 (Epitheliall), Mouse primary tumor
Description: MYD88 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 345 amino acids (1-309 a.a) and having a molecular mass of 38.7kDa.;MYD88 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Ovary primary carcinoma with matched metastatic carcinoma tissue array
Description: A competitive ELISA for quantitative measurement of Human Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Myd88/ Myeloid differentiation primary response protein MyD88 ELISA Kit
Description: A competitive ELISA for quantitative measurement of Mouse Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human MYD88/ Myeloid differentiation primary response protein MyD88 ELISA Kit
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Myeloid differentiation primary response protein MyD88 in samples from serum, plasma, tissue homogenates and other biological fluids.
ELISA kit for Mouse Myeloid differentiation primary response protein MyD88
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Myeloid differentiation primary response protein MyD88 in samples from serum, plasma, tissue homogenates and other biological fluids.
Western blot Kit for G. pig Primary Antibodies, Chemilum. Substrate
System and liver tissue cultured described here can serve as a better in vitro Platform culture of 3D and basic unit for varied applications, including drug development, research hepatocyte polarity, the design of the bioreactor liver bioartificial, and network and construction of organs for tissue engineering heart or liver cholestasis injury.