Clinically relevant CYP3A4 induction mechanisms and drug screening in 3D spheroid cultures of primary human hepatocytes.

CYP3A4 induction is an important cause of drug-drug interactions, making early identification of drug candidates with the obligations CYP3A4 induction in drug development prerequisites. Here, we present a 3D spheroid cultures of primary human hepatocytes (PHH) as CYP3A4 induction of new screening models.

Screening of 25 drugs (12 known inducers of CYP3A4 in vivo and 13 negative control) at physiologically relevant concentrations revealed 100% sensitivity and 100% specificity system. Three of inducers of CYP3A4 in vivo induction of CYP3A4 displayed capacity is much higher in comparison with the 3D spherical culture in 2D monolayer culture. Among them, we identify AZD1208, PIM kinase inhibitor in Phase I development stopped because of unexpected autoinduction CYP3A4, CYP3A4 inducer as the only active in 3D spheroids but not in 2D monolayer culture.

Gene knockdown experiments revealed that AZD1208 require PXR to induce CYP3A4. Rifampicin require PXR solely to induce CYP3A4 and CYP2B6, while phenobarbital-mediated induction of CYPs is not indicative of the absolute dependence on both PXR or CAR demonstrated its ability to switch the activation of nuclear receptors. Mechanistic Study of AZD1208 to find involvement of MAPK / ERK pathway in induction of CYP3A4-sensitive culture format used, as revealed by the inhibition of ERK1 / 2 phosphorylation Tyr204 and sensitivity to EGF pressure. Accordingly, we also identified lapatinib, a dual EGFR / HER2 inhibitor, such as CYP3A4 inducer active just another round in 3D culture.

Our findings offer insight into the pathways involved in the induction of CYP3A4 and suggest spheroids PHH for preclinical screening of CYP3A4 induction.

Clinically relevant CYP3A4 induction mechanisms and drug screening in 3D spheroid cultures of primary human hepatocytes.
Clinically relevant CYP3A4 induction mechanisms and drug screening in 3D spheroid cultures of primary human hepatocytes.

Glucose and lipid metabolism screening models of hepatocyte spheroids after culture by injecting fiber fragments.

With the increase in obesity, diabetes and other metabolic diseases, in vitro liver cell and tissue models play an important role in the identification of active pharmaceutical ingredients. Up to now three-dimensional (3D) models rarely focuses on the culture of hepatic glucose and lipid metabolism.

In addition, primary human liver cells suffer from limited availability and differences among donors to build the model reproduced. Thus, in this study, the most available human liver cancer cells (HepG2), and primary hepatocytes from rat (RPH) proposed to build a 3D spheroids using fiber fragments graft syringe with galactose (GSF) as a substrate. RPH and spheroids showed HepG2 cells and cell fragments strong-fiber interaction to promote cell survival, albumin and urea synthesis.

Compared with HepG2 spheroids, spheroids RPH demonstrate strong abilities in terms of consumption of glucose metabolism of glucose, glycogen intracellular content, the level of glucose in gluconeogenesis and sensitivity to the hormone modulators such as insulin and glucagon. On the other hand, HepG2 spheroids displays a strong ability of lipid metabolism in produce significantly higher levels of total cholesterol and triglycerides.

Compared with those who did not fragment the fiber, the GSF-supported 3D culture establish an effective model for in vitro glucose (RPH spheroids) and lipid metabolism (HepG2 spheroids). The model confirmed screening of each enzyme activity and gene expression and showed significantly higher sensitivity and clinically relevant response to hypoglycemic and lipid-lowering drugs.

Human Hepatocytes

HC4230 500,000 Cells
EUR 826.00

Liver Dissociation System 2 (Hepatocytes, Parenchymal hepatocytes), Mouse and Rat

4-20302 ea Ask for price

Anti-Hepatocytes antibody

STJ16100176 100 µg
EUR 389.00

Liver Dissociation System 4 (Hepatocytes, rat), Rat

4-20304 ea Ask for price

Mouse Liver PrimaCell: Hepatocytes

2-82100 1 Kit Ask for price

Human Liver PrimaCell: Hepatocytes

2-96121 1 Kit Ask for price

Human Hepatocytes - Alcohol Addicted

HC4230AA 500,000 Cells
EUR 957.00

Human Hepatocytes - Opioid Addicted

HC4230OP 500000
EUR 957.00

Immortalized Human Hepatocytes - hTERT

T0058 1x106 cells / 1.0 ml Ask for price

Immortalized Human Hepatocytes - Ras

T0059 1x106 cells / 1.0 ml Ask for price

Immortalized Monkey Hepatocytes-SV40

T0073 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Hepatocytes - SV40

T0101 1x106 cells / 1.0 ml Ask for price

Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)

RAT-5 1
EUR 1138.00

Rat Liver PrimaCell: Normal Hepatocytes Growth Medium

9-25095 5 x 100 ml Ask for price

Rat Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80322 1 x 100 ml Ask for price

Immortalized Mouse Hepatocytes-Conditionally (ImHep)

T0099 1x106 cells / 1.0 ml Ask for price

Liver Dissociation System 1 (Hepatocytes), Mouse

4-20301 ea Ask for price

Immortalized Hamster Hepatocytes-SV40 T+t

T0071 1x106 cells / 1.0 ml Ask for price

Immortalized Monkey Hepatocytes-SV40 T+t

T0074 1x106 cells / 1.0 ml Ask for price

Rat of the Wistar strain, 22 organs, 66 cores (1.5mm), set 1

ART661 1
EUR 250.00
Description: Normal tissues of 22 major organs triplicated from 3 Wistar strain rats, set 1, non-overlapping with ART662

Rat of the Wistar strain, 22 organs, 66 cores (1.5mm), set 2

ART662 1
EUR 250.00
Description: Normal tissues of 22 major organs triplicated from 3 Wistar strain rats, set 2, non-overlapping with ART661

Liver Dissociation System 6 (Hepatocytes,Nonparenchymal), Mouse and Rat

4-20306 ea Ask for price

TnI (Primary) Antibody

abx018056-100ug 100 ug
EUR 342.00
  • Shipped within 5-10 working days.

Primary Human Neurons

HNC001 300,000 Cyroperserved Cells
EUR 1223.00

Rat Primary hepatic carcinoma ELISA kit

E02P0656-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Primary hepatic carcinoma ELISA kit

E02P0656-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Primary hepatic carcinoma ELISA kit

E02P0656-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Liver Dissociation System 3 (Hepatocytes, Kupffer, Parenchymal), Mouse and Rat

4-20303 ea Ask for price

Mouse Liver PrimaCell: Normal Hepatocytes Growth Medium

9-32100 5 x 100 ml Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Medium

9-46121 5 x 100 ml Ask for price

Mouse Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80306 1 x 100 ml Ask for price

Human Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80314 1 x 100 ml Ask for price

Antigen-Antibody Pen For Rat Primary antibodies

PEN-R10 1
EUR 202.00

DB Primary Antibody diluent

DBD-125 125 ml
EUR 150.00

DB Primary Antibody diluent

DBD-250 250 ml
EUR 245.00

DB Primary Antibody diluent

DB-D-125 125 ml
EUR 150.00

DB Primary Antibody diluent

DB-D-250 250 ml
EUR 245.00

Primary Antibody Dropper Vial

PAV015 1 ea.
EUR 53.00

Rat Membrane primary amine oxidase(AOC3) ELISA kit

CSB-EL001855RA-24T 1 plate of 24 wells
EUR 165.00
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Membrane primary amine oxidase (AOC3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Membrane primary amine oxidase(AOC3) ELISA kit

1-CSB-EL001855RA
  • EUR 703.00
  • EUR 4843.00
  • EUR 2570.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Membrane primary amine oxidase(AOC3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-82522 1 Kit Ask for price

Rat Aoc3/ Membrane primary amine oxidase ELISA Kit

E0075Ra 1 Kit
EUR 571.00

Rat Aoc3(Membrane primary amine oxidase) ELISA Kit

ER0434 96T
EUR 567.60
  • Detection range: 0.78-50 ng/ml
  • Uniprot ID: O08590
  • Alias: Aoc3/VAP-1/HPAO/SSAO/Copper amine oxidase/HPAOSSAO/Semicarbazide-sensitive amine oxidase/VAP1/membrane primary amine oxidase/Vascular adhesion protein 1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.469 ng/ml

Rat Myeloid differentiation primary response protein MyD88, Myd8

ELI-05538r 96 Tests
EUR 886.00

ELISA kit for Rat Membrane primary amine oxidase

EK2706 96 tests
EUR 553.00
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Membrane primary amine oxidase in samples from serum, plasma, tissue homogenates and other biological fluids.

Primary Antibody Diluent (Tris, Green)

ATG-20000 20 L
EUR 1177.00

Primary Antibody Diluent (Tris, Green)

ATG125 125 ml
EUR 74.00

Primary Antibody Diluent (Tris, Green)

ATG500 500 ml
EUR 101.00

Primary Antibody Diluent (Tris, Green)

ATG999 1000 ml
EUR 124.00

Primary Antibody Diluent (Phosphate, Green)

APG125 125 ml
EUR 74.00

Primary Antibody Diluent (Phosphate, Green)

APG500 500 ml
EUR 101.00

Primary Antibody Diluent (Phosphate, Green)

APG999 1000 ml
EUR 124.00

Primary Antibody Diluent (Phosphate, Green)

APG010 10 L
EUR 671.00

Western blot Kit for Rat Primary Antibodies, Chemilum. Substrate

80203-Rt 1 kit
EUR 651.00

Rat Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-26095 1 Set Ask for price

Membrane Primary Amine Oxidase (AOC3) Antibody

20-abx333876
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Myeloid Differentiation Primary Response 88 Protein

20-abx261025
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Membrane Primary Amine Oxidase (AOC3) Antibody

abx033988-400ul 400 ul
EUR 523.00
  • Shipped within 5-10 working days.

Membrane Primary Amine Oxidase (AOC3) Antibody

abx033988-80l 80 µl
EUR 286.00
  • Shipped within 5-10 working days.

Membrane Primary Amine Oxidase (AOC3) Antibody

20-abx001627
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Western Blot Primary Antibody Diluent Buffer

abx090689-100ml 100 ml
EUR 182.00
  • Shipped within 5-10 working days.

Myeloid Differentiation Primary Response 88 Antibody

20-abx137458
  • EUR 704.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Membrane Primary Amine Oxidase (AOC3) Antibody

20-abx110946
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Human Primary hepatic carcinoma ELISA kit

E01P0656-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Primary hepatic carcinoma ELISA kit

E01P0656-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Primary hepatic carcinoma ELISA kit

E01P0656-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Primary hepatic carcinoma ELISA kit

E07P0656-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Primary hepatic carcinoma ELISA kit

E07P0656-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Primary hepatic carcinoma ELISA kit

E07P0656-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit

E04P0656-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit

E04P0656-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit

E04P0656-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit

E06P0656-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit

E06P0656-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit

E06P0656-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit

E03P0656-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit

E03P0656-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit

E03P0656-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit

E09P0656-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit

E09P0656-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit

E09P0656-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit

E08P0656-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit

E08P0656-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit

E08P0656-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Breast PrimaCell: Normal Mammary Epithelial Primary Cells Growth Medium

9-25022 5 x 100 ml Ask for price

Rat Breast Tissue Preparation Buffer: Normal Mammary Epithelial Primary Cells

9-80229 1 x 100 ml Ask for price

Rat Myd88/ Myeloid differentiation primary response protein MyD88 ELISA Kit

E0652Ra 1 Kit
EUR 571.00

Rat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit

E02M0411-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit

E02M0411-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit

E02M0411-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Aoc3 ELISA Kit| Rat Membrane primary amine oxidase ELISA Kit

EF017277 96 Tests
EUR 689.00

Membrane Primary Amine Oxidase (AOC3) Antibody (HRP)

20-abx336489
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Membrane Primary Amine Oxidase (AOC3) Antibody (FITC)

20-abx336490
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Membrane Primary Amine Oxidase (AOC3) Antibody (Biotin)

20-abx336491
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Human primary hepatic carcinoma,PHC ELISA Kit

201-12-1673 96 tests
EUR 440.00
  • This primary hepatic carcinoma ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Antigen-Antibody Pen For Chicken Primary antibodies

PEN-C5 1
EUR 202.00

Antigen-Antibody Pen For Goat Primary antibodies

PEN-G3 1
EUR 202.00

Antigen-Antibody Pen For Human Primary antibodies

PEN-H7 1
EUR 202.00

Antigen-Antibody Pen For Mouse Primary antibodies

PEN-M2 1
EUR 202.00

Antigen-Antibody Pen For Rabbit Primary antibodies

PEN-R1 1
EUR 202.00

Antigen-Antibody Pen For Sheep Primary antibodies

PEN-S4 1
EUR 202.00

Antigen-Antibody Pen For Hamster Primary antibodies

PEN-T8 1
EUR 202.00

Human 293, transformed primary embryonal kidney lysate

HCL-1210 100ug
EUR 213.00

Human primary hepatic carcinoma(PHC)ELISA Kit

GA-E1689HM-48T 48T
EUR 289.00

Human primary hepatic carcinoma(PHC)ELISA Kit

GA-E1689HM-96T 96T
EUR 466.00

Porcine Myeloid differentiation primary response protein MyD88,

ELI-05540p 96 Tests
EUR 928.00

Guinea pig Primary hepatic carcinoma ELISA kit

E05P0656-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Primary hepatic carcinoma ELISA kit

E05P0656-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Primary hepatic carcinoma ELISA kit

E05P0656-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human primary hepatic carcinoma(PHC)ELISA Kit

QY-E03406 96T
EUR 361.00

Human Myeloid differentiation primary response protein MyD88 (MYD88)

1-CSB-EP859945HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 37.2 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Myeloid differentiation primary response protein MyD88(MYD88) expressed in E.coli

Human Membrane primary amine oxidase(AOC3) ELISA kit

CSB-EL001855HU-24T 1 plate of 24 wells
EUR 165.00
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Membrane primary amine oxidase (AOC3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Membrane primary amine oxidase(AOC3) ELISA kit

1-CSB-EL001855HU
  • EUR 703.00
  • EUR 4843.00
  • EUR 2570.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Membrane primary amine oxidase(AOC3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Membrane primary amine oxidase(AOC3) ELISA kit

CSB-EL001855MO-24T 1 plate of 24 wells
EUR 165.00
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Mouse Membrane primary amine oxidase (AOC3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Membrane primary amine oxidase(AOC3) ELISA kit

1-CSB-EL001855MO
  • EUR 703.00
  • EUR 4843.00
  • EUR 2570.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Mouse Membrane primary amine oxidase(AOC3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-82021 1 Kit Ask for price

Human Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-96023 1 Kit Ask for price

Mammary Dissociation System 5 (Epitheliall), Mouse primary tumor

4-20325 ea Ask for price

Mouse Aoc3/ Membrane primary amine oxidase ELISA Kit

E0103Mo 1 Kit
EUR 571.00

Antigen-Antibody Pen For G. Pig Primary antibodies

PEN-P6 1
EUR 202.00

Mouse Aoc3(Membrane primary amine oxidase) ELISA Kit

EM0513 96T
EUR 567.60
  • Detection range: 31.2-2000 pg/ml
  • Uniprot ID: O70423
  • Alias: Aoc3/AOC3/VAP-1/HPAO/SSAO/Copper amine oxidase/HPAOSSAO/Semicarbazide-sensitive amine oxidase/VAP1/membrane primary amine oxidase/Vascular adhesion protein 1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 18.75pg/ml

Human AOC3/ Membrane primary amine oxidase ELISA Kit

E2737Hu 1 Kit
EUR 537.00

Myd88 ELISA Kit| Mouse Myeloid differentiation primary response

EF013284 96 Tests
EUR 689.00

Human AOC3(Membrane primary amine oxidase ) ELISA Kit

EH0577 96T
EUR 524.10
  • Detection range: 1.56-100 ng/ml
  • Uniprot ID: Q16853
  • Alias: AOC3/VAP-1/HPAO/SSAO/Copper amine oxidase/HPAOSSAO/Semicarbazide-sensitive amine oxidase/VAP1/membrane primary amine oxidase/Vascular adhesion protein 1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml

Mouse Membrane primary amine oxidase, Aoc3 ELISA KIT

ELI-03528m 96 Tests
EUR 865.00

Human Membrane primary amine oxidase, AOC3 ELISA KIT

ELI-03529h 96 Tests
EUR 824.00

Bovine Membrane primary amine oxidase, AOC3 ELISA KIT

ELI-03531b 96 Tests
EUR 928.00

Human Myeloid differentiation primary response protein MyD88, MY

ELI-05535h 96 Tests
EUR 824.00

Bovine Myeloid differentiation primary response protein MyD88, M

ELI-05536b 96 Tests
EUR 928.00

ELISA kit for Mouse Membrane primary amine oxidase

EK2704 96 tests
EUR 553.00
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Membrane primary amine oxidase in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Human Membrane primary amine oxidase

EK2705 96 tests
EUR 452.00
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Membrane primary amine oxidase in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-33100 1 Set Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-47121 1 Set Ask for price

Western blot Kit for Rabbit Primary Antibodies, Chemilum. Substrate

80200-Rb 1 kit
EUR 651.00

Western blot Kit for Goat Primary Antibodies, Chemilum. Substrate

80201-Gt 1 kit
EUR 651.00

Western blot Kit for Mouse Primary Antibodies, Chemilum. Substrate

80202-Mo 1 kit
EUR 651.00

Thus, the culture configuration shows the predicted in vitro platform for defining the profiles of glucose and lipid metabolism and screening of therapeutic agents for metabolic disorders such as diabetes and obesity.