3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber

IPSC human hepatocytes derived great promise as a source of cells for cell therapy and drug screening. However, the hepatocyte culture method for highly-quantitative unassigned. Here, we have developed a 3D encapsulation and cultivation methods for IPSC-hepatocytes in microfiber hydrogel core-shell (aka cell fiber).

3D microenvironment in the form of fibers composed of abundant extracellular matrix (ECM), the IPSC-liver hepatocytes exhibited many characteristics, including albumin secretion and gene expression markers of liver (ALB, HNF4α, ASGPR1, CYP2C19, and CYP3A4). In addition, we found that the fibers are mechanically stable and can apply for hepatocyte transplantation.

Three days after transplantation of microfiber into the abdominal cavity immunodeficient mice, human albumin was detected in the peripheral blood of mice transplanted. These results indicate that fiber-hepatocytes promising IPSC well as in vitro models for drug screening or as graft implantation for treating heart failure.

Visualization and isolation of murine hepatocytes special zones which maintain the expression of different cytochrome P450 oxidase in primary culture

parenchymal hepatocytes are responsible for most of the metabolic function of the liver, but showing a different functional properties depending on their localization in the liver lobule. P450 oxidase is a family of drug-metabolism enzyme, which is expressed primarily in hepatocytes local in centrilobular regions (zone 3). This paper describes a unique strain of transgenic mice that distinguishes zone 3 hepatocytes of periportal zone 1 hepatocytes by EGFP fluorescence intensity.

The second zone 1 and zone 3 hepatocytes isolated from these mice showed the zone-specific gene expression patterns were the same as in the liver tissue in vivo.

Experiments using primary cultures of hepatocytes demonstrated that the combination of low oxygen concentration and activation of Wnt / β-catenin expression zones maintain drug-metabolism enzyme 3-specific P450, marked by their susceptibility to acetaminophen-induced mitochondrial dysfunction. Hepatocytes special zone provides a useful system in the pathophysiology of liver research and drug development.

3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber
3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber

Erythromycin evaluation as a tool to assess the contribution of CYP3A low clearance compound in hepatocyte cultures long term

Long-term cultures of hepatocytes as HepatoPac® system is suitable for evaluating the metabolic turnover low clearance (CL) for sustained drug metabolism capacity and their long-term survival. Erythromycin (ERY), moderate CYP3A inhibitors based mechanisms, evaluated as a tool in HepatoPac models to assess the contribution of CYP3A for clearance drug candidates.

ERY inhibit CYP3A activity by 58 and 80% for the 3 and 10 pM, respectively, up to 72 hours. At 30 pM, ERY MDZ hydroxylation is inhibited by> 85% for the entire duration of the incubation of 144 hours. Clint APZ inhibited 58% by 3 pM ERY, 75% by 15 pM ERY, 89% by 30 pM ERY and 94% by 60 pM ERY. ERY (30 M) did not significantly affect Clint substrate for several other major CYP isoforms was evaluated and no real inhibit UGT isoforms 1A1, 1A3, 1A4, 1A6, 1A9, 2B7 or 2B15 were assessed using recombinant UGTs.

ERY is only slightly increased CYP3A4 gene expression 2.1-fold (14% of RIF induction) at 120 pM indicates that the effective concentration for the inhibition of CYP3A activity (30-60 uM), AHR (arylhydrocarbon receptor), CAR (constitutive androstane receptor ) and PXR (pregnane X receptor) activation is not possible to increase the apparent levels of an enzyme or other metabolic drug transporter.

Syrian Hamster IgG Isotype Control

SIGGPP5.5-25 25 µg
EUR 426.91

Syrian Hamster IgG Isotype Control

SIGGPU-500 500 µg
EUR 514.27

Immortalized Hamster Hepatocytes-SV40 T+t

T0071 1x106 cells / 1.0 ml Ask for price

Hamster IgG, purified (Syrian, isotype control)

20003-1 1 mg
EUR 169.2

Hamster (Syrian) IgG Isotype Control (FITC)

abx405033-01mg 0.1 mg
EUR 477.6

Hamster IgG-Biotin conjugate, isotype control (Syrian)

20003-1-B 100 test
EUR 196.8

Hamster IgG-Cy5 conjugate, isotype control (Syrian)

20003-1-Cy5 50 tests
EUR 270

Hamster IgG-FITC conjugate, isotype control (Syrian)

20003-1-F 100 tests
EUR 196.8

Hamster IgG-HRP conjugate, isotype control (Syrian)

20003-1-HP 100 tests
EUR 196.8

Purified Hamster (syrian) serum albumin protein pure

ALBHS-15-N 1 mg
EUR 270

Goat Anti-Hamster IgG (H+L)-HRP conjugate (Syrian) IgG (Syrian) IgG

80320 1 ml
EUR 242.4

Goat anti Syrian Hamster IgG (H + L) (biotin)

43R-GH001 1 ml
EUR 511.2
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (biotin)

Goat anti Syrian Hamster IgG (H + L) (FITC)

43R-ID035ft 1 mg
EUR 453.6
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (FITC)

Goat anti Syrian Hamster IgG (H + L) (HRP)

43R-IG038hrp 1 ml
EUR 510
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (HRP)

Goat anti Syrian Hamster IgG (H + L) (rhodamine)

43R-IG097rd 1 mg
EUR 411.6
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (rhodamine)

Hamster IgG-R-PE conjugate, isotype control (Syrian)

20003-1-PE 50 tests
EUR 270

Hamster (Syrian) control serum (non-immunized) Mixed breed

NGHS-1 1 ml
EUR 123.6

Hamster (Syrian) control serum (non-immunized) Mixed breed

NGHS-5 5 ml
EUR 242.4

Antigen-Antibody Pen For Hamster Primary antibodies

PEN-T8 1
EUR 242.4

Hamster IgG-R-PE-Cy5.5 conjugate, isotype control (Syrian)

20003-1-PC5 50 tests
EUR 300

Syrian Hamster Anti-Mouse CD28 Monoclonal antibody, clone 37.51

CABT-L4358-1mg 1 mg
EUR 1076.4

Syrian Hamster Anti-Mouse CD28 Monoclonal antibody, clone 37.51

CABT-L4358-5mg 5 mg
EUR 2886

Hamster (Syrian) control serum (non-immunized, delipidized) Mixed breed

NGHS-5D 5 ml
EUR 270

Human Hepatocytes

HC4230 500,000 Cells
EUR 991.2

Goat Anti-Hamster IgG (H+L) aff pure, unlabeled (Syrian)

80319 1 mg
EUR 242.4

Goat Anti-Hamster (Syrian) IgG (H+L)-Alk. Phosphatase conjugate

80322 1 ml
EUR 270

Goat Anti-Hamster IgG (H+L)-Biotin conjugate Syrian IgG,

80340 0.5 ml
EUR 242.4

Syrian Hamster Anti-Mouse Podoplanin (gp38) Monoclonal antibody, clone 8.1.1

CABT-L4294-1mg 1 mg
EUR 1076.4

Syrian Hamster Anti-Mouse Podoplanin (gp38) Monoclonal antibody, clone 8.1.1

CABT-L4294-5mg 5 mg
EUR 2886

Syrian Hamster Anti-Mouse KLRG-1 Monoclonal antibody, clone 2F1

CABT-L4349-1mg 1 mg
EUR 1076.4

Syrian Hamster Anti-Mouse KLRG-1 Monoclonal antibody, clone 2F1

CABT-L4349-5mg 5 mg
EUR 2886

Hamster (Syrian) control serum (non-immunized, heat-inactivated) Mixed breed

NGHS-5H 5 ml
EUR 270

Syrian Hamster Anti-Mouse 4-1BB (CD137) Monoclonal antibody, clone 17B5

CABT-L4524-1mg 1 mg
EUR 1076.4

Syrian Hamster Anti-Mouse 4-1BB (CD137) Monoclonal antibody, clone 17B5

CABT-L4524-5mg 5 mg
EUR 2886

Anti-Hepatocytes antibody

STJ16100176 100 µg
EUR 466.8

Hamster (Syrian) control serum (non-immunized, delipidized, and heat inactivated) Mixed breed

NGHS-5HD 5 ml
EUR 315.6

Golden Shiner virus PCR kit

PCR-V271-48R 50T
EUR 987.6

Golden Shiner virus PCR kit

PCR-V271-96R 100T
EUR 1335.6

Liver Dissociation System 2 (Hepatocytes, Parenchymal hepatocytes), Mouse and Rat

4-20302 ea Ask for price

Mouse Liver PrimaCell: Hepatocytes

2-82100 1 Kit Ask for price

Rat Liver PrimaCell: Hepatocytes

2-82595 1 Kit Ask for price

Human Liver PrimaCell: Hepatocytes

2-96121 1 Kit Ask for price

Human Hepatocytes - Alcohol Addicted

HC4230AA 500,000 Cells
EUR 1148.4

Human Hepatocytes - Opioid Addicted

HC4230OP 500000
EUR 1148.4

Immortalized Human Hepatocytes - hTERT

T0058 1x106 cells / 1.0 ml Ask for price

Immortalized Human Hepatocytes - Ras

T0059 1x106 cells / 1.0 ml Ask for price

Immortalized Monkey Hepatocytes-SV40

T0073 1x106 cells / 1.0 ml Ask for price

Immortalized Rat Hepatocytes - SV40

T0078 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Hepatocytes - SV40

T0101 1x106 cells / 1.0 ml Ask for price

Goat Anti-Hamster IgG (H+L)-Rhodamine (TRITC) conjugate (Syrian, Gadsorbed with other species)

80331 0.5 ml
EUR 255.6

Goat Anti-Hamster IgG (H+L)-Cy3 conjugate (Syrian IgG, adsorbed with other species)

80334 0.5 ml
EUR 328.8

Goat Anti-Hamster IgG (H+L)-Cy5 conjugate (Syrian IgG, adsorbed with other species)

80335 0.5 ml
EUR 328.8

Golden Shiner virus RT PCR kit

RTq-V271-100R 100T
EUR 1573.2

Golden Shiner virus RT PCR kit

RTq-V271-150R 150T
EUR 2144.4

Golden Shiner virus RT PCR kit

RTq-V271-50R 50T
EUR 1155.6

Primary UTI

MED1270 PK10
EUR 10.99

Immortalized Mouse Hepatocytes-Conditionally (ImHep)

T0099 1x106 cells / 1.0 ml Ask for price

Goat Anti-Hamster IgG (H+L)-Texas Red conjugate (Syrian IgG, adsorbed with other species)

80333 0.5 ml
EUR 255.6

Hamster (Armenian Hamster) Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80180-AH 1 kit
EUR 781.2

Golden Shiner virus One-Step PCR kit

Oneq-V271-100R 100T
EUR 1932

Golden Shiner virus One-Step PCR kit

Oneq-V271-150R 150T
EUR 2646

Golden Shiner virus One-Step PCR kit

Oneq-V271-50R 50T
EUR 1410

TnI (Primary) Antibody

abx018056-100ug 100 ug
EUR 410.4

Primary Human Neurons

HNC001 300,000 Cyroperserved Cells
EUR 1467.6

Primary UTI Opaque

MED1272 PK10
EUR 11.81

Primary UTI Agar

MED1334 EACH
EUR 573.42

Primary Opalescence Solution

EPPOS01 100ML
EUR 98.04

Human Primary Chondrocytes

171-T0020 5 Cells
EUR 2760

Primary malignant melanoma

ME204 each
EUR 534
Description: Primary malignant melanoma, metastatic malignant melanoma and skin tissue array, incluing TNM /Stage, 68 cases/204 cores (core size 1.0mm),replacing ME208

Liver Dissociation System 1 (Hepatocytes), Mouse

4-20301 ea Ask for price

Immortalized Monkey Hepatocytes-SV40 T+t

T0074 1x106 cells / 1.0 ml Ask for price

Hamster IgM

31C-CH0707 1 mg
EUR 492
Description: Purified Hamster IgM

Liver Dissociation System 4 (Hepatocytes, rat), Rat

4-20304 ea Ask for price

Rat Liver PrimaCell: Normal Hepatocytes Growth Medium

9-25095 5 x 100 ml Ask for price

Mouse Liver PrimaCell: Normal Hepatocytes Growth Medium

9-32100 5 x 100 ml Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Medium

9-46121 5 x 100 ml Ask for price

Mouse Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80306 1 x 100 ml Ask for price

Human Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80314 1 x 100 ml Ask for price

Rat Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80322 1 x 100 ml Ask for price

DB Primary Antibody diluent

DB-D-125 125 ml
EUR 180

DB Primary Antibody diluent

DB-D-250 250 ml
EUR 294

DB Primary Antibody diluent

DBD-125 125 ml
EUR 180

DB Primary Antibody diluent

DBD-250 250 ml
EUR 294

Primary Antibody Dropper Vial

PAV015 1 ea.
EUR 63.6

Primary Blue Solution 100ml

EPBS01 100ML
EUR 98.04

Primary Red Solution 100ml

EPRS01 100ML
EUR 145.92

Hamster Mono Anti-Mouse CD3e IgG (Hamster IgG)

MCD003E-M 100 Tests
EUR 578.4

Hamster Mono Anti-Mouse CD3z IgG (Hamster IgG)

MCD003Z-M 100 ug
EUR 578.4

Hamster IgG (FITC)

65C-CH0702 1 mg
EUR 240
Description: Purified Hamster IgG FITC conjugate

Hamster IgG (HRP)

65C-CH0703 1 mg
EUR 296.4
Description: Purified Hamster IgG HRP conjugate

Hamster Ig fraction

31R-1009 10 mg
EUR 165.6
Description: Hamster Ig fraction control or blocking reagent

Hamster Lymphocyte antibody

20R-LR011 20 mg
EUR 392.4
Description: Rabbit polyclonal Hamster Lymphocyte antibody

Hamster Lymphocyte antibody

20R-LR012 2 ml
EUR 289.2
Description: Rabbit polyclonal Hamster Lymphocyte antibody

Hamster RBC antibody

20R-RR021 20 mg
EUR 392.4
Description: Rabbit polyclonal Hamster RBC antibody

Hamster RBC antibody

20R-RR022 5 mg
EUR 664.8
Description: Rabbit polyclonal Hamster RBC antibody

Hamster Complement Serum

32R-CH001 5 ml
EUR 508.8
Description: Purified normal Hamster Complement isolated from blood

Hamster CD28 Antibody

abx139999-01mg 0.1 mg
EUR 343.2

Hamster CD3 Antibody

abx140000-01mg 0.1 mg
EUR 343.2

Hamster CD3 Antibody

abx140001-01mg 0.1 mg
EUR 343.2

Hamster CD80 Antibody

abx140014-01mg 0.1 mg
EUR 343.2

Hamster CD154 Antibody

abx140091-01mg 0.1 mg
EUR 410.4

Hamster CD154 Antibody

abx140092-01mg 0.1 mg
EUR 343.2

Hamster Helios Antibody

abx140094-01mg 0.1 mg
EUR 493.2

Recombinant Hamster Uter

P0138 100ug
EUR 626.83
Description: Recombinant Hamster protein for Uter

Hamster Transthyretin (TTR)

QY-E90097 96T
EUR 573.6

Primary Antibody Diluent (Phosphate, Green)

APG125 125 ml
EUR 88.8

Primary Antibody Diluent (Phosphate, Green)

APG500 500 ml
EUR 121.2

Primary Antibody Diluent (Phosphate, Green)

APG999 1000 ml
EUR 148.8

Primary Antibody Diluent (Tris, Green)

ATG-20000 20 L
EUR 1412.4

Primary Antibody Diluent (Tris, Green)

ATG125 125 ml
EUR 88.8

Primary Antibody Diluent (Tris, Green)

ATG500 500 ml
EUR 121.2

Primary Antibody Diluent (Tris, Green)

ATG999 1000 ml
EUR 148.8

Primary Antibody Diluent (Phosphate, Green)

APG010 10 L
EUR 805.2

MicroMolar Primary Amine Assay Kit

PAA100K 100 assays
EUR 168.93
Description: This product includes 0.5 ml of 10 x PPA dye, 5.5 ml of Assay buffer, 5 ml of reagent A and 0.05 ml of 1 mM methylamine-HCl. It is for measurement of 100 samples using 96-well plates. Cuvettes may also be used for measurements.

Liver Dissociation System 6 (Hepatocytes,Nonparenchymal), Mouse and Rat

4-20306 ea Ask for price

Goat Anti-Hamster IgG (H+L)-HRP conjugate (Syrian IgG, adsorbed with Bv, Ch, Gt, Gp, Hs, Mo, Rb, Rt, Sh IgGs)

80339 0.5 ml
EUR 270

ERY at concentrations up to 60 pM non-toxic to 6 days of incubation. Use of ERY to selectively inhibit CYP3A in hepatocytes, long-term high-functioning as HepatoPac can be a valuable strategy to evaluate the contribution