3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber

IPSC human hepatocytes derived great promise as a source of cells for cell therapy and drug screening. However, the hepatocyte culture method for highly-quantitative unassigned. Here, we have developed a 3D encapsulation and cultivation methods for IPSC-hepatocytes in microfiber hydrogel core-shell (aka cell fiber).

3D microenvironment in the form of fibers composed of abundant extracellular matrix (ECM), the IPSC-liver hepatocytes exhibited many characteristics, including albumin secretion and gene expression markers of liver (ALB, HNF4α, ASGPR1, CYP2C19, and CYP3A4). In addition, we found that the fibers are mechanically stable and can apply for hepatocyte transplantation.

Three days after transplantation of microfiber into the abdominal cavity immunodeficient mice, human albumin was detected in the peripheral blood of mice transplanted. These results indicate that fiber-hepatocytes promising IPSC well as in vitro models for drug screening or as graft implantation for treating heart failure.

Visualization and isolation of murine hepatocytes special zones which maintain the expression of different cytochrome P450 oxidase in primary culture

parenchymal hepatocytes are responsible for most of the metabolic function of the liver, but showing a different functional properties depending on their localization in the liver lobule. P450 oxidase is a family of drug-metabolism enzyme, which is expressed primarily in hepatocytes local in centrilobular regions (zone 3). This paper describes a unique strain of transgenic mice that distinguishes zone 3 hepatocytes of periportal zone 1 hepatocytes by EGFP fluorescence intensity.

The second zone 1 and zone 3 hepatocytes isolated from these mice showed the zone-specific gene expression patterns were the same as in the liver tissue in vivo.

Experiments using primary cultures of hepatocytes demonstrated that the combination of low oxygen concentration and activation of Wnt / β-catenin expression zones maintain drug-metabolism enzyme 3-specific P450, marked by their susceptibility to acetaminophen-induced mitochondrial dysfunction. Hepatocytes special zone provides a useful system in the pathophysiology of liver research and drug development.

3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber
3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber

Erythromycin evaluation as a tool to assess the contribution of CYP3A low clearance compound in hepatocyte cultures long term

Long-term cultures of hepatocytes as HepatoPac® system is suitable for evaluating the metabolic turnover low clearance (CL) for sustained drug metabolism capacity and their long-term survival. Erythromycin (ERY), moderate CYP3A inhibitors based mechanisms, evaluated as a tool in HepatoPac models to assess the contribution of CYP3A for clearance drug candidates.

ERY inhibit CYP3A activity by 58 and 80% for the 3 and 10 pM, respectively, up to 72 hours. At 30 pM, ERY MDZ hydroxylation is inhibited by> 85% for the entire duration of the incubation of 144 hours. Clint APZ inhibited 58% by 3 pM ERY, 75% by 15 pM ERY, 89% by 30 pM ERY and 94% by 60 pM ERY. ERY (30 M) did not significantly affect Clint substrate for several other major CYP isoforms was evaluated and no real inhibit UGT isoforms 1A1, 1A3, 1A4, 1A6, 1A9, 2B7 or 2B15 were assessed using recombinant UGTs.

ERY is only slightly increased CYP3A4 gene expression 2.1-fold (14% of RIF induction) at 120 pM indicates that the effective concentration for the inhibition of CYP3A activity (30-60 uM), AHR (arylhydrocarbon receptor), CAR (constitutive androstane receptor ) and PXR (pregnane X receptor) activation is not possible to increase the apparent levels of an enzyme or other metabolic drug transporter.

Syrian Hamster IgG Isotype Control

SIGGPP5.5-25 25 µg
EUR 355.76

Syrian Hamster IgG Isotype Control

SIGGPU-500 500 µg
EUR 428.56

Immortalized Hamster Hepatocytes-SV40 T+t

T0071 1x106 cells / 1.0 ml Ask for price

Hamster IgG, purified (Syrian, isotype control)

20003-1 1 mg
EUR 141

Hamster (Syrian) IgG Isotype Control (FITC)

abx405033-01mg 0.1 mg
EUR 398

Hamster IgG-Biotin conjugate, isotype control (Syrian)

20003-1-B 100 test
EUR 164

Hamster IgG-Cy5 conjugate, isotype control (Syrian)

20003-1-Cy5 50 tests
EUR 225

Hamster IgG-FITC conjugate, isotype control (Syrian)

20003-1-F 100 tests
EUR 164

Hamster IgG-HRP conjugate, isotype control (Syrian)

20003-1-HP 100 tests
EUR 164

Purified Hamster (syrian) serum albumin protein pure

ALBHS-15-N 1 mg
EUR 225

Goat Anti-Hamster IgG (H+L)-HRP conjugate (Syrian) IgG (Syrian) IgG

80320 1 ml
EUR 202

Hamster IgG-R-PE conjugate, isotype control (Syrian)

20003-1-PE 50 tests
EUR 225

Hamster (Syrian) control serum (non-immunized) Mixed breed

NGHS-1 1 ml
EUR 103

Hamster (Syrian) control serum (non-immunized) Mixed breed

NGHS-5 5 ml
EUR 202

Goat anti Syrian Hamster IgG (H + L) (biotin)

43R-GH001 1 ml
EUR 426
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (biotin)

Goat anti Syrian Hamster IgG (H + L) (FITC)

43R-ID035ft 1 mg
EUR 378
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (FITC)

Goat anti Syrian Hamster IgG (H + L) (HRP)

43R-IG038hrp 1 ml
EUR 425
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (HRP)

Goat anti Syrian Hamster IgG (H + L) (rhodamine)

43R-IG097rd 1 mg
EUR 343
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (rhodamine)

Hamster IgG-R-PE-Cy5.5 conjugate, isotype control (Syrian)

20003-1-PC5 50 tests
EUR 250

Goat Anti-Hamster IgG (H+L)-FITC conjugate (Syrian)

80325 1 ml
EUR 213

Syrian Hamster Anti-Mouse CD28 Monoclonal antibody, clone 37.51

CABT-L4358-1mg 1 mg
EUR 897

Syrian Hamster Anti-Mouse CD28 Monoclonal antibody, clone 37.51

CABT-L4358-5mg 5 mg
EUR 2405

Hamster (Syrian) control serum (non-immunized, delipidized) Mixed breed

NGHS-5D 5 ml
EUR 225

Antigen-Antibody Pen For Hamster Primary antibodies

PEN-T8 1
EUR 202

Human Hepatocytes

HC4230 500,000 Cells
EUR 826

Goat Anti-Hamster IgG (H+L) aff pure, unlabeled (Syrian)

80319 1 mg
EUR 202

Goat Anti-Hamster (Syrian) IgG (H+L)-Alk. Phosphatase conjugate

80322 1 ml
EUR 225

Goat Anti-Hamster IgG (H+L)-Biotin conjugate Syrian IgG,

80340 0.5 ml
EUR 202

Syrian Hamster Anti-Mouse Podoplanin (gp38) Monoclonal antibody, clone 8.1.1

CABT-L4294-1mg 1 mg
EUR 897

Syrian Hamster Anti-Mouse Podoplanin (gp38) Monoclonal antibody, clone 8.1.1

CABT-L4294-5mg 5 mg
EUR 2405

Syrian Hamster Anti-Mouse KLRG-1 Monoclonal antibody, clone 2F1

CABT-L4349-1mg 1 mg
EUR 897

Syrian Hamster Anti-Mouse KLRG-1 Monoclonal antibody, clone 2F1

CABT-L4349-5mg 5 mg
EUR 2405

Hamster (Syrian) control serum (non-immunized, heat-inactivated) Mixed breed

NGHS-5H 5 ml
EUR 225

Anti-Hepatocytes antibody

STJ16100176 100 µg
EUR 389

Syrian Hamster Anti-Mouse 4-1BB (CD137) Monoclonal antibody, clone 17B5

CABT-L4524-1mg 1 mg
EUR 897

Syrian Hamster Anti-Mouse 4-1BB (CD137) Monoclonal antibody, clone 17B5

CABT-L4524-5mg 5 mg
EUR 2405

Golden Shiner virus PCR kit

PCR-V271-48R 50T
EUR 823

Golden Shiner virus PCR kit

PCR-V271-96R 100T
EUR 1113

Hamster (Syrian) control serum (non-immunized, delipidized, and heat inactivated) Mixed breed

NGHS-5HD 5 ml
EUR 263

Liver Dissociation System 2 (Hepatocytes, Parenchymal hepatocytes), Mouse and Rat

4-20302 ea Ask for price

Human Hepatocytes - Alcohol Addicted

HC4230AA 500,000 Cells
EUR 957

Human Hepatocytes - Opioid Addicted

HC4230OP 500000
EUR 957

Mouse Liver PrimaCell: Hepatocytes

2-82100 1 Kit Ask for price

Rat Liver PrimaCell: Hepatocytes

2-82595 1 Kit Ask for price

Human Liver PrimaCell: Hepatocytes

2-96121 1 Kit Ask for price

Immortalized Human Hepatocytes - hTERT

T0058 1x106 cells / 1.0 ml Ask for price

Immortalized Human Hepatocytes - Ras

T0059 1x106 cells / 1.0 ml Ask for price

Immortalized Monkey Hepatocytes-SV40

T0073 1x106 cells / 1.0 ml Ask for price

Immortalized Rat Hepatocytes - SV40

T0078 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Hepatocytes - SV40

T0101 1x106 cells / 1.0 ml Ask for price

Golden Shiner virus RT PCR kit

RTq-V271-100R 100T
EUR 1311

Golden Shiner virus RT PCR kit

RTq-V271-150R 150T
EUR 1787

Golden Shiner virus RT PCR kit

RTq-V271-50R 50T
EUR 963

Goat Anti-Hamster IgG (H+L)-Rhodamine (TRITC) conjugate (Syrian, Gadsorbed with other species)

80331 0.5 ml
EUR 213

Goat Anti-Hamster IgG (H+L)-Cy3 conjugate (Syrian IgG, adsorbed with other species)

80334 0.5 ml
EUR 274

Goat Anti-Hamster IgG (H+L)-Cy5 conjugate (Syrian IgG, adsorbed with other species)

80335 0.5 ml
EUR 274

Immortalized Mouse Hepatocytes-Conditionally (ImHep)

T0099 1x106 cells / 1.0 ml Ask for price

Golden Shiner virus One-Step PCR kit

Oneq-V271-100R 100T
EUR 1610

Golden Shiner virus One-Step PCR kit

Oneq-V271-150R 150T
EUR 2205

Golden Shiner virus One-Step PCR kit

Oneq-V271-50R 50T
EUR 1175

Goat Anti-Hamster IgG (H+L)-Texas Red conjugate (Syrian IgG, adsorbed with other species)

80333 0.5 ml
EUR 213

Hamster (Armenian Hamster) Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80180-AH 1 kit
EUR 651

Liver Dissociation System 1 (Hepatocytes), Mouse

4-20301 ea Ask for price

Immortalized Monkey Hepatocytes-SV40 T+t

T0074 1x106 cells / 1.0 ml Ask for price

Primary Human Neurons

HNC001 300,000 Cyroperserved Cells
EUR 1223

TnI (Primary) Antibody

abx018056-100ug 100 ug
EUR 342

Rat Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80322 1 x 100 ml Ask for price

Liver Dissociation System 4 (Hepatocytes, rat), Rat

4-20304 ea Ask for price

Rat Liver PrimaCell: Normal Hepatocytes Growth Medium

9-25095 5 x 100 ml Ask for price

Mouse Liver PrimaCell: Normal Hepatocytes Growth Medium

9-32100 5 x 100 ml Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Medium

9-46121 5 x 100 ml Ask for price

Mouse Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80306 1 x 100 ml Ask for price

Human Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80314 1 x 100 ml Ask for price

Hamster IgM

31C-CH0707 1 mg
EUR 410
Description: Purified Hamster IgM

Primary Antibody Dropper Vial

PAV015 1 ea.
EUR 53

DB Primary Antibody diluent

DBD-125 125 ml
EUR 150

DB Primary Antibody diluent

DBD-250 250 ml
EUR 245

DB Primary Antibody diluent

DB-D-125 125 ml
EUR 150

DB Primary Antibody diluent

DB-D-250 250 ml
EUR 245

Hamster Mono Anti-Mouse CD3e IgG (Hamster IgG)

MCD003E-M 100 Tests
EUR 482

Hamster Mono Anti-Mouse CD3z IgG (Hamster IgG)

MCD003Z-M 100 ug
EUR 482

Hamster CD154 Antibody

abx140092-01mg 0.1 mg
EUR 286

Hamster Helios Antibody

abx140094-01mg 0.1 mg
EUR 411

Hamster CD28 Antibody

abx139999-01mg 0.1 mg
EUR 286

Hamster CD3 Antibody

abx140000-01mg 0.1 mg
EUR 286

Hamster CD3 Antibody

abx140001-01mg 0.1 mg
EUR 286

Hamster CD80 Antibody

abx140014-01mg 0.1 mg
EUR 286

Hamster CD154 Antibody

abx140091-01mg 0.1 mg
EUR 342

Hamster Transthyretin (TTR)

QY-E90097 96T
EUR 478

Recombinant Hamster Uter

P0138 100ug
EUR 522.36
Description: Recombinant Hamster protein for Uter

Hamster IgG (FITC)

65C-CH0702 1 mg
EUR 200
Description: Purified Hamster IgG FITC conjugate

Hamster IgG (HRP)

65C-CH0703 1 mg
EUR 247
Description: Purified Hamster IgG HRP conjugate

Hamster Lymphocyte antibody

20R-LR011 20 mg
EUR 327
Description: Rabbit polyclonal Hamster Lymphocyte antibody

Hamster Lymphocyte antibody

20R-LR012 2 ml
EUR 241
Description: Rabbit polyclonal Hamster Lymphocyte antibody

Hamster RBC antibody

20R-RR021 20 mg
EUR 327
Description: Rabbit polyclonal Hamster RBC antibody

Hamster RBC antibody

20R-RR022 5 mg
EUR 554
Description: Rabbit polyclonal Hamster RBC antibody

Hamster Ig fraction

31R-1009 10 mg
EUR 138
Description: Hamster Ig fraction control or blocking reagent

Hamster Complement Serum

32R-CH001 5 ml
EUR 424
Description: Purified normal Hamster Complement isolated from blood

Liver Dissociation System 6 (Hepatocytes,Nonparenchymal), Mouse and Rat

4-20306 ea Ask for price

Primary Antibody Diluent (Phosphate, Green)

APG010 10 L
EUR 671

Primary Antibody Diluent (Phosphate, Green)

APG125 125 ml
EUR 74

Primary Antibody Diluent (Phosphate, Green)

APG500 500 ml
EUR 101

Primary Antibody Diluent (Phosphate, Green)

APG999 1000 ml
EUR 124

Primary Antibody Diluent (Tris, Green)

ATG-20000 20 L
EUR 1177

Primary Antibody Diluent (Tris, Green)

ATG125 125 ml
EUR 74

Primary Antibody Diluent (Tris, Green)

ATG500 500 ml
EUR 101

Primary Antibody Diluent (Tris, Green)

ATG999 1000 ml
EUR 124

Goat Anti-Hamster IgG (H+L)-HRP conjugate (Syrian IgG, adsorbed with Bv, Ch, Gt, Gp, Hs, Mo, Rb, Rt, Sh IgGs)

80339 0.5 ml
EUR 225

Hamster Mono Anti-Mouse CD25 , Pur. Low Endotoxin (Hamster IgG)

MCD027-M 100 ug
EUR 482

Liver Dissociation System 3 (Hepatocytes, Kupffer, Parenchymal), Mouse and Rat

4-20303 ea Ask for price

Antibody for Hamster HSP110

SPC-195C 0.025mg
EUR 174
Description: A polyclonal antibody for HSP110 from Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) . The antibody is produced in rabbit after immunization with Hamster Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:100). This HSP110 antibody is unconjugated.

Antibody for Hamster HSP110

SPC-195D 0.1mg
EUR 306
Description: A polyclonal antibody for HSP110 from Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) . The antibody is produced in rabbit after immunization with Hamster Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:100). This HSP110 antibody is unconjugated.

Antibody for Hamster HSP110

SPC-195D-A390 0.1mg
EUR 353
Description: A polyclonal antibody for HSP110 from Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) . The antibody is produced in rabbit after immunization with Hamster Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:100). This HSP110 antibody is conjugated to ATTO 390.

Antibody for Hamster HSP110

SPC-195D-A488 0.1mg
EUR 352
Description: A polyclonal antibody for HSP110 from Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) . The antibody is produced in rabbit after immunization with Hamster Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:100). This HSP110 antibody is conjugated to ATTO 488.

Antibody for Hamster HSP110

SPC-195D-A565 0.1mg
EUR 352
Description: A polyclonal antibody for HSP110 from Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) . The antibody is produced in rabbit after immunization with Hamster Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:100). This HSP110 antibody is conjugated to ATTO 565.

Antibody for Hamster HSP110

SPC-195D-A594 0.1mg
EUR 352
Description: A polyclonal antibody for HSP110 from Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) . The antibody is produced in rabbit after immunization with Hamster Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:100). This HSP110 antibody is conjugated to ATTO 594.

ERY at concentrations up to 60 pM non-toxic to 6 days of incubation. Use of ERY to selectively inhibit CYP3A in hepatocytes, long-term high-functioning as HepatoPac can be a valuable strategy to evaluate the contribution