3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber

IPSC human hepatocytes derived great promise as a source of cells for cell therapy and drug screening. However, the hepatocyte culture method for highly-quantitative unassigned. Here, we have developed a 3D encapsulation and cultivation methods for IPSC-hepatocytes in microfiber hydrogel core-shell (aka cell fiber).

3D microenvironment in the form of fibers composed of abundant extracellular matrix (ECM), the IPSC-liver hepatocytes exhibited many characteristics, including albumin secretion and gene expression markers of liver (ALB, HNF4α, ASGPR1, CYP2C19, and CYP3A4). In addition, we found that the fibers are mechanically stable and can apply for hepatocyte transplantation.

Three days after transplantation of microfiber into the abdominal cavity immunodeficient mice, human albumin was detected in the peripheral blood of mice transplanted. These results indicate that fiber-hepatocytes promising IPSC well as in vitro models for drug screening or as graft implantation for treating heart failure.

Visualization and isolation of murine hepatocytes special zones which maintain the expression of different cytochrome P450 oxidase in primary culture

parenchymal hepatocytes are responsible for most of the metabolic function of the liver, but showing a different functional properties depending on their localization in the liver lobule. P450 oxidase is a family of drug-metabolism enzyme, which is expressed primarily in hepatocytes local in centrilobular regions (zone 3). This paper describes a unique strain of transgenic mice that distinguishes zone 3 hepatocytes of periportal zone 1 hepatocytes by EGFP fluorescence intensity.

The second zone 1 and zone 3 hepatocytes isolated from these mice showed the zone-specific gene expression patterns were the same as in the liver tissue in vivo.

Experiments using primary cultures of hepatocytes demonstrated that the combination of low oxygen concentration and activation of Wnt / β-catenin expression zones maintain drug-metabolism enzyme 3-specific P450, marked by their susceptibility to acetaminophen-induced mitochondrial dysfunction. Hepatocytes special zone provides a useful system in the pathophysiology of liver research and drug development.

3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber
3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber

Erythromycin evaluation as a tool to assess the contribution of CYP3A low clearance compound in hepatocyte cultures long term

Long-term cultures of hepatocytes as HepatoPac® system is suitable for evaluating the metabolic turnover low clearance (CL) for sustained drug metabolism capacity and their long-term survival. Erythromycin (ERY), moderate CYP3A inhibitors based mechanisms, evaluated as a tool in HepatoPac models to assess the contribution of CYP3A for clearance drug candidates.

ERY inhibit CYP3A activity by 58 and 80% for the 3 and 10 pM, respectively, up to 72 hours. At 30 pM, ERY MDZ hydroxylation is inhibited by> 85% for the entire duration of the incubation of 144 hours. Clint APZ inhibited 58% by 3 pM ERY, 75% by 15 pM ERY, 89% by 30 pM ERY and 94% by 60 pM ERY. ERY (30 M) did not significantly affect Clint substrate for several other major CYP isoforms was evaluated and no real inhibit UGT isoforms 1A1, 1A3, 1A4, 1A6, 1A9, 2B7 or 2B15 were assessed using recombinant UGTs.

ERY is only slightly increased CYP3A4 gene expression 2.1-fold (14% of RIF induction) at 120 pM indicates that the effective concentration for the inhibition of CYP3A activity (30-60 uM), AHR (arylhydrocarbon receptor), CAR (constitutive androstane receptor ) and PXR (pregnane X receptor) activation is not possible to increase the apparent levels of an enzyme or other metabolic drug transporter.

Syrian Hamster IgG Isotype Control

SIGGB-200 200 µg
EUR 472.24

Syrian Hamster IgG Isotype Control

SIGGF-200 200 µg
EUR 545.04

Syrian Hamster IgG Isotype Control

SIGGPE-100 100 µg
EUR 515.92

Syrian Hamster IgG Isotype Control

SIGGPP5.5-100 100 µg
EUR 676.08

Syrian Hamster IgG Isotype Control

SIGGPP5.5-25 25 µg
EUR 355.76

Syrian Hamster IgG Isotype Control

SIGGPU-500 500 µg
EUR 428.56

Immortalized Hamster Hepatocytes-SV40 T+t

T0071 1x106 cells / 1.0 ml Ask for price

Hamster (Syrian) IgG Isotype Control (FITC)

abx405033-01mg 0.1 mg
EUR 398.00
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Hamster IgG, purified (Syrian, isotype control)

20003-1 1 mg
EUR 141.00

Hamster IgG-Biotin conjugate, isotype control (Syrian)

20003-1-B 100 test
EUR 164.00

Hamster IgG-Cy5 conjugate, isotype control (Syrian)

20003-1-Cy5 50 tests
EUR 225.00

Hamster IgG-FITC conjugate, isotype control (Syrian)

20003-1-F 100 tests
EUR 164.00

Hamster IgG-HRP conjugate, isotype control (Syrian)

20003-1-HP 100 tests
EUR 164.00

Purified Hamster (syrian) serum albumin protein pure

ALBHS-15-N 1 mg
EUR 225.00

Goat Anti-Hamster IgG (H+L)-HRP conjugate (Syrian) IgG (Syrian) IgG

80320 1 ml
EUR 202.00

Hamster IgG-R-PE conjugate, isotype control (Syrian)

20003-1-PE 50 tests
EUR 225.00

Goat anti Syrian Hamster IgG (H + L) (biotin)

43R-GH001 1 ml
EUR 426.00
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (biotin)

Goat anti Syrian Hamster IgG (H + L) (FITC)

43R-ID035ft 1 mg
EUR 378.00
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (FITC)

Goat anti Syrian Hamster IgG (H + L) (HRP)

43R-IG038hrp 1 ml
EUR 425.00
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (HRP)

Goat anti Syrian Hamster IgG (H + L) (rhodamine)

43R-IG097rd 1 mg
EUR 343.00
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (rhodamine)

Hamster (Syrian) control serum (non-immunized) Mixed breed

NGHS-1 1 ml
EUR 103.00

Hamster (Syrian) control serum (non-immunized) Mixed breed

NGHS-5 5 ml
EUR 202.00

Antigen-Antibody Pen For Hamster Primary antibodies

PEN-T8 1
EUR 202.00

Goat Anti-Hamster IgG (H+L)-FITC conjugate (Syrian)

80325 1 ml
EUR 213.00

Hamster IgG-R-PE-Cy5.5 conjugate, isotype control (Syrian)

20003-1-PC5 50 tests
EUR 250.00

Syrian Hamster Anti-Mouse CD28 Monoclonal antibody, clone 37.51

CABT-L4358-5mg 5 mg
EUR 2405.00

Syrian Hamster Anti-Mouse CD28 Monoclonal antibody, clone 37.51

CABT-L4358-1mg 1 mg
EUR 897.00

Hamster (Syrian) control serum (non-immunized, delipidized) Mixed breed

NGHS-5D 5 ml
EUR 225.00

Goat Anti-Hamster IgG (H+L) aff pure, unlabeled (Syrian)

80319 1 mg
EUR 202.00

Goat Anti-Hamster (Syrian) IgG (H+L)-Alk. Phosphatase conjugate

80322 1 ml
EUR 225.00

Goat Anti-Hamster IgG (H+L)-Biotin conjugate Syrian IgG,

80340 0.5 ml
EUR 202.00

Syrian Hamster Anti-Mouse Podoplanin (gp38) Monoclonal antibody, clone 8.1.1

CABT-L4294-1mg 1 mg
EUR 897.00

Syrian Hamster Anti-Mouse Podoplanin (gp38) Monoclonal antibody, clone 8.1.1

CABT-L4294-5mg 5 mg
EUR 2405.00

Syrian Hamster Anti-Mouse KLRG-1 Monoclonal antibody, clone 2F1

CABT-L4349-5mg 5 mg
EUR 2405.00

Syrian Hamster Anti-Mouse KLRG-1 Monoclonal antibody, clone 2F1

CABT-L4349-1mg 1 mg
EUR 897.00

Hamster (Syrian) control serum (non-immunized, heat-inactivated) Mixed breed

NGHS-5H 5 ml
EUR 225.00

Human Hepatocytes

HC4230 500,000 Cells
EUR 826.00

Syrian Hamster Anti-Mouse 4-1BB (CD137) Monoclonal antibody, clone 17B5

CABT-L4524-1mg 1 mg
EUR 897.00

Syrian Hamster Anti-Mouse 4-1BB (CD137) Monoclonal antibody, clone 17B5

CABT-L4524-5mg 5 mg
EUR 2405.00

Hamster (Syrian) control serum (non-immunized, delipidized, and heat inactivated) Mixed breed

NGHS-5HD 5 ml
EUR 263.00

Anti-Hepatocytes antibody

STJ16100176 100 µg
EUR 389.00

Golden Shiner virus PCR kit

PCR-V271-48R 50T
EUR 524.40
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Golden Shiner virus

Golden Shiner virus PCR kit

PCR-V271-96R 100T
EUR 669.40
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Description: An conventional PCR kit for detection of Golden Shiner virus

Goat Anti-Hamster IgG (H+L)-Rhodamine (TRITC) conjugate (Syrian, Gadsorbed with other species)

80331 0.5 ml
EUR 213.00

Goat Anti-Hamster IgG (H+L)-Cy3 conjugate (Syrian IgG, adsorbed with other species)

80334 0.5 ml
EUR 274.00

Goat Anti-Hamster IgG (H+L)-Cy5 conjugate (Syrian IgG, adsorbed with other species)

80335 0.5 ml
EUR 274.00

Liver Dissociation System 2 (Hepatocytes, Parenchymal hepatocytes), Mouse and Rat

4-20302 ea Ask for price

Mouse Liver PrimaCell: Hepatocytes

2-82100 1 Kit Ask for price

Rat Liver PrimaCell: Hepatocytes

2-82595 1 Kit Ask for price

Human Liver PrimaCell: Hepatocytes

2-96121 1 Kit Ask for price

Human Hepatocytes - Alcohol Addicted

HC4230AA 500,000 Cells
EUR 957.00

Human Hepatocytes - Opioid Addicted

HC4230OP 500000
EUR 957.00

Immortalized Human Hepatocytes - hTERT

T0058 1x106 cells / 1.0 ml Ask for price

Immortalized Human Hepatocytes - Ras

T0059 1x106 cells / 1.0 ml Ask for price

Immortalized Monkey Hepatocytes-SV40

T0073 1x106 cells / 1.0 ml Ask for price

Immortalized Rat Hepatocytes - SV40

T0078 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Hepatocytes - SV40

T0101 1x106 cells / 1.0 ml Ask for price

Goat Anti-Hamster IgG (H+L)-Texas Red conjugate (Syrian IgG, adsorbed with other species)

80333 0.5 ml
EUR 213.00

Hamster (Armenian Hamster) Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80180-AH 1 kit
EUR 651.00

Golden Shiner virus RT PCR kit

RTq-V271-100R 100T
EUR 776.40
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Golden Shiner virus .

Golden Shiner virus RT PCR kit

RTq-V271-150R 150T
EUR 877.90
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Golden Shiner virus .

Golden Shiner virus RT PCR kit

RTq-V271-50R 50T
EUR 631.40
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Golden Shiner virus .

Immortalized Mouse Hepatocytes-Conditionally (ImHep)

T0099 1x106 cells / 1.0 ml Ask for price

Golden Shiner virus One-Step PCR kit

Oneq-V271-100R 100T
EUR 933.00
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Golden Shiner virus.

Golden Shiner virus One-Step PCR kit

Oneq-V271-150R 150T
EUR 1060.60
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Golden Shiner virus.

Golden Shiner virus One-Step PCR kit

Oneq-V271-50R 50T
EUR 751.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Golden Shiner virus.

Hamster IgM

31C-CH0707 1 mg
EUR 410.00
Description: Purified Hamster IgM

Liver Dissociation System 1 (Hepatocytes), Mouse

4-20301 ea Ask for price

Immortalized Monkey Hepatocytes-SV40 T+t

T0074 1x106 cells / 1.0 ml Ask for price

TnI (Primary) Antibody

abx018056-100ug 100 ug
EUR 342.00
  • Shipped within 5-10 working days.

Primary Human Neurons

HNC001 300,000 Cyroperserved Cells
EUR 1223.00

Hamster Mono Anti-Mouse CD3e IgG (Hamster IgG)

MCD003E-M 100 Tests
EUR 482.00

Hamster Mono Anti-Mouse CD3z IgG (Hamster IgG)

MCD003Z-M 100 ug
EUR 482.00

Hamster IgG (FITC)

65C-CH0702 1 mg
EUR 200.00
Description: Purified Hamster IgG FITC conjugate

Hamster IgG (HRP)

65C-CH0703 1 mg
EUR 247.00
Description: Purified Hamster IgG HRP conjugate

Hamster Complement Serum

32R-CH001 5 ml
EUR 424.00
Description: Purified normal Hamster Complement isolated from blood

Hamster Lymphocyte antibody

20R-LR011 20 mg
EUR 327.00
Description: Rabbit polyclonal Hamster Lymphocyte antibody

Hamster Lymphocyte antibody

20R-LR012 2 ml
EUR 241.00
Description: Rabbit polyclonal Hamster Lymphocyte antibody

Hamster RBC antibody

20R-RR021 20 mg
EUR 327.00
Description: Rabbit polyclonal Hamster RBC antibody

Hamster RBC antibody

20R-RR022 5 mg
EUR 554.00
Description: Rabbit polyclonal Hamster RBC antibody

Hamster Ig fraction

31R-1009 10 mg
EUR 138.00
Description: Hamster Ig fraction control or blocking reagent

Hamster CD28 Antibody

abx139999-01mg 0.1 mg
EUR 286.00
  • Shipped within 5-12 working days.

Hamster CD3 Antibody

abx140000-01mg 0.1 mg
EUR 286.00
  • Shipped within 5-12 working days.

Hamster CD3 Antibody

abx140001-01mg 0.1 mg
EUR 286.00
  • Shipped within 5-12 working days.

Hamster CD80 Antibody

abx140014-01mg 0.1 mg
EUR 286.00
  • Shipped within 5-12 working days.

Hamster CD154 Antibody

abx140091-01mg 0.1 mg
EUR 342.00
  • Shipped within 5-12 working days.

Hamster CD154 Antibody

abx140092-01mg 0.1 mg
EUR 286.00
  • Shipped within 5-12 working days.

Hamster Helios Antibody

abx140094-01mg 0.1 mg
EUR 411.00
  • Shipped within 5-12 working days.

Recombinant Hamster Uter

P0138 100ug
EUR 522.36
  • Formulation: pH7.4, Lyophilized from a 0.2um filtered solution in PBS with 5% trehalose
  • Reconstitution: Sterile distilled water
  • Purity: Greater than 95% by SDS-PAGE gel analyses
  • Uniprot ID: Q8VD96
Description: Recombinant Hamster protein for Uter

Hamster Transthyretin (TTR)

QY-E90097 96T
EUR 478.00

Rat Liver PrimaCell: Normal Hepatocytes Growth Medium

9-25095 5 x 100 ml Ask for price

Mouse Liver PrimaCell: Normal Hepatocytes Growth Medium

9-32100 5 x 100 ml Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Medium

9-46121 5 x 100 ml Ask for price

Mouse Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80306 1 x 100 ml Ask for price

Human Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80314 1 x 100 ml Ask for price

Rat Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80322 1 x 100 ml Ask for price

Liver Dissociation System 4 (Hepatocytes, rat), Rat

4-20304 ea Ask for price

DB Primary Antibody diluent

DBD-125 125 ml
EUR 150.00

DB Primary Antibody diluent

DBD-250 250 ml
EUR 245.00

DB Primary Antibody diluent

DB-D-125 125 ml
EUR 150.00

DB Primary Antibody diluent

DB-D-250 250 ml
EUR 245.00

Primary Antibody Dropper Vial

PAV015 1 ea.
EUR 53.00

Hamster Mono Anti-Mouse CD25 , Pur. Low Endotoxin (Hamster IgG)

MCD027-M 100 ug
EUR 482.00

Hamster Red Blood Cells

88R-H001 5 ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Hamster Red Blood Cells

Hamster Red Blood Cells

88R-H003 10 ml
EUR 489.00
Description: Washed and freshly prepared 10% suspension of Hamster Red Blood Cells

Hamster Lymphocyte antibody (FITC)

60R-LR001FT 2 mg
EUR 365.00
Description: Rabbit polyclonal Hamster Lymphocyte antibody (FITC) conjugated

Hamster RBC antibody (FITC)

60R-RR021FT 3 mg
EUR 392.00
Description: Rabbit polyclonal Hamster RBC antibody (FITC) conjugated

Genomic DNA - Hamster Male

D1H34999-G01 100 ug
EUR 188.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Female

D1H34999-G02 100 ug
EUR 188.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Hamster Anti CD28 Antibody

20-abx137056
  • EUR 356.00
  • EUR 523.00
  • 0.5 mg
  • 1 mg
  • Shipped within 5-10 working days.

Hamster CD3 Antibody (PerCP)

abx140002-01mg 0.1 mg
EUR 398.00
  • Shipped within 5-12 working days.

Hamster CD3 Antibody (PE)

abx140003-01mg 0.1 mg
EUR 328.00
  • Shipped within 5-12 working days.

Hamster CD3 Antibody (APC)

abx140004-01mg 0.1 mg
EUR 370.00
  • Shipped within 5-12 working days.

Hamster CD3 Antibody (Biotin)

abx140005-01mg 0.1 mg
EUR 272.00
  • Shipped within 5-12 working days.

Hamster CD3 Antibody (FITC)

abx140006-01mg 0.1 mg
EUR 314.00
  • Shipped within 5-12 working days.

Hamster CD154 Antibody (FITC)

abx140093-01mg 0.1 mg
EUR 537.00
  • Shipped within 5-12 working days.

ERY at concentrations up to 60 pM non-toxic to 6 days of incubation. Use of ERY to selectively inhibit CYP3A in hepatocytes, long-term high-functioning as HepatoPac can be a valuable strategy to evaluate the contribution