3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber

IPSC human hepatocytes derived great promise as a source of cells for cell therapy and drug screening. However, the hepatocyte culture method for highly-quantitative unassigned. Here, we have developed a 3D encapsulation and cultivation methods for IPSC-hepatocytes in microfiber hydrogel core-shell (aka cell fiber).

3D microenvironment in the form of fibers composed of abundant extracellular matrix (ECM), the IPSC-liver hepatocytes exhibited many characteristics, including albumin secretion and gene expression markers of liver (ALB, HNF4α, ASGPR1, CYP2C19, and CYP3A4). In addition, we found that the fibers are mechanically stable and can apply for hepatocyte transplantation.

Three days after transplantation of microfiber into the abdominal cavity immunodeficient mice, human albumin was detected in the peripheral blood of mice transplanted. These results indicate that fiber-hepatocytes promising IPSC well as in vitro models for drug screening or as graft implantation for treating heart failure.

Visualization and isolation of murine hepatocytes special zones which maintain the expression of different cytochrome P450 oxidase in primary culture

parenchymal hepatocytes are responsible for most of the metabolic function of the liver, but showing a different functional properties depending on their localization in the liver lobule. P450 oxidase is a family of drug-metabolism enzyme, which is expressed primarily in hepatocytes local in centrilobular regions (zone 3). This paper describes a unique strain of transgenic mice that distinguishes zone 3 hepatocytes of periportal zone 1 hepatocytes by EGFP fluorescence intensity.

The second zone 1 and zone 3 hepatocytes isolated from these mice showed the zone-specific gene expression patterns were the same as in the liver tissue in vivo.

Experiments using primary cultures of hepatocytes demonstrated that the combination of low oxygen concentration and activation of Wnt / β-catenin expression zones maintain drug-metabolism enzyme 3-specific P450, marked by their susceptibility to acetaminophen-induced mitochondrial dysfunction. Hepatocytes special zone provides a useful system in the pathophysiology of liver research and drug development.

3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber
3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber

Erythromycin evaluation as a tool to assess the contribution of CYP3A low clearance compound in hepatocyte cultures long term

Long-term cultures of hepatocytes as HepatoPac® system is suitable for evaluating the metabolic turnover low clearance (CL) for sustained drug metabolism capacity and their long-term survival. Erythromycin (ERY), moderate CYP3A inhibitors based mechanisms, evaluated as a tool in HepatoPac models to assess the contribution of CYP3A for clearance drug candidates.

ERY inhibit CYP3A activity by 58 and 80% for the 3 and 10 pM, respectively, up to 72 hours. At 30 pM, ERY MDZ hydroxylation is inhibited by> 85% for the entire duration of the incubation of 144 hours. Clint APZ inhibited 58% by 3 pM ERY, 75% by 15 pM ERY, 89% by 30 pM ERY and 94% by 60 pM ERY. ERY (30 M) did not significantly affect Clint substrate for several other major CYP isoforms was evaluated and no real inhibit UGT isoforms 1A1, 1A3, 1A4, 1A6, 1A9, 2B7 or 2B15 were assessed using recombinant UGTs.

ERY is only slightly increased CYP3A4 gene expression 2.1-fold (14% of RIF induction) at 120 pM indicates that the effective concentration for the inhibition of CYP3A activity (30-60 uM), AHR (arylhydrocarbon receptor), CAR (constitutive androstane receptor ) and PXR (pregnane X receptor) activation is not possible to increase the apparent levels of an enzyme or other metabolic drug transporter.

Golden Syrian Hamster IgG (F(ab)2 specific) rabbit polyclonal antibody, Biotin

R1328B 1 mg Ask for price

Golden Syrian Hamster IgG (F(ab)2 specific) rabbit polyclonal antibody, Texas Red

R1328TR 1 mg Ask for price

Golden Syrian Hamster IgG (F(ab)2 specific) rabbit polyclonal antibody, Aff - Purified

R1328P 5 mg Ask for price

Immortalized Hamster Hepatocytes (SV40)

T0072 1x10^6 cells / 1.0 ml Ask for price

Primary Dog (Beagle) Hepatocytes, Plateable

T0118 5x10^5 cells / 1.0 ml
EUR 1375

Primary Rat (Fischer) Hepatocytes, Plateable

T0108 5x10^5 cells / 1.0 ml
EUR 1375

Primary Dog (Beagle) Hepatocytes, Suspension

T0117 5x10^5 cells / 1.0 ml
EUR 1375

Primary Rat (Fischer) Hepatocytes, Suspension

T0107 5x10^5 cells / 1.0 ml
EUR 1375

Normal Human Primary Plateable Hepatocytes

T5539 1x10^6 cells / 1.0 ml
EUR 925

Normal Human Primary Plateable Hepatocytes

T5541 1x10^6 cells / 1.0 ml Ask for price

Normal Human Primary Suspension Hepatocytes

T5538 1x10^6 cells / 1.0 ml Ask for price

Primary Mouse (CD-1) Hepatocytes, Plateable

T0120 5x10^5 cells / 1.0 ml
EUR 1375

Primary Mouse (CD-1) Hepatocytes, Suspension

T0119 5x10^5 cells / 1.0 ml
EUR 1375

Immortalized Hamster Hepatocytes-SV40 T+t

T0071 1x106 cells / 1.0 ml Ask for price

Primary Rat (Wistar Han) Hepatocytes, Plateable

T0106 5x10^5 cells / 1.0 ml
EUR 1375

Primary Mouse (C57BI/6) Hepatocytes, Plateable

T0122 5x10^5 cells / 1.0 ml
EUR 1375

Primary Monkey (Cynomolgus) hepatocytes, Plateable

T0112 5x10^5 cells / 1.0 ml
EUR 1375

Primary Rat (Wistar Han) Hepatocytes, Suspension

T0105 5x10^5 cells / 1.0 ml
EUR 1375

Primary Mouse (C57BI/6) Hepatocytes, Suspension

T0121 5x10^5 cells / 1.0 ml
EUR 1375

Enhanced Primary Human Hepatocytes - 5 million

T5996 5x10^6 cells / 1.0 ml Ask for price

Primary Monkey (Cynomolgus) Hepatocytes, Suspension

T0111 5x10^5 cells / 1.0 ml
EUR 1375

Primary Minipig (Gottingen) Hepatocytes, Suspension

T0113 5x10^5 cells / 1.0 ml
EUR 1375

Primary Rat (Sprague Dawley) Hepatocytes, Plateable

T0104 5x10^5 cells / 1.0 ml
EUR 1375

Primary Rat (Sprague Dawley) Hepatocytes, Suspension

T0103 5x10^5 cells / 1.0 ml
EUR 1375

Enhanced Primary Human Hepatocytes – 1 Million

T5995 1x10^6 cells / 1.0ml Ask for price

Primary Human Hepatocytes, Transporter Uptake Qualified

T0125 5x10^5 cells / 1.0 ml
EUR 3500

Primary Human Hepatocytes, Induction Qualified, Plateable

T0128 5x10^5 cells / 1.0 ml
EUR 2625

Primary Human Hepatocytes, Metabolism Qualified, Plateable

T0124 5x10^5 cells / 1.0 ml
EUR 2625

Primary Human Hepatocytes, Metabolism Qualified, Suspension

T0123 5x10^5 cells / 1.0 ml
EUR 1375

Primary Rabbit (New Zealand White) Hepatocytes, Suspension

T0115 5x10^5 cells / 1.0 ml
EUR 1375

*Hamster Syrian Gold IgG ELISA Kit

IHMSGIGGKT each
EUR 554
Description: *Hamster Syrian Gold IgG ELISA Kit

*Hamster Syrian Gold IgG Affinity Purified

IHMSGIGG10MG each
EUR 138
Description: *Hamster Syrian Gold IgG Affinity Purified

*Hamster Syrian Gold IgG Affinity Purified

IHMSGIGG50MG each
EUR 300
Description: *Hamster Syrian Gold IgG Affinity Purified

Innovative Grade US Origin Hamster Syrian Gold Brain

IGHMSGBR each
EUR 104
Description: Innovative Grade US Origin Hamster Syrian Gold Brain

Innovative Grade US Origin Hamster Syrian Gold Heart

IGHMSGHT each
EUR 104
Description: Innovative Grade US Origin Hamster Syrian Gold Heart

Innovative Grade US Origin Hamster Syrian Gold Liver

IGHMSGLR each
EUR 104
Description: Innovative Grade US Origin Hamster Syrian Gold Liver

Innovative Grade US Origin Hamster Syrian Gold Serum

IGHMSGSER100ML each
EUR 1240
Description: Innovative Grade US Origin Hamster Syrian Gold Serum

Innovative Grade US Origin Hamster Syrian Gold Serum

IGHMSGSER10ML each
EUR 149
Description: Innovative Grade US Origin Hamster Syrian Gold Serum

Innovative Grade US Origin Hamster Syrian Gold Serum

IGHMSGSER25ML each
EUR 349
Description: Innovative Grade US Origin Hamster Syrian Gold Serum

Innovative Grade US Origin Hamster Syrian Gold Serum

IGHMSGSER50ML each
EUR 659
Description: Innovative Grade US Origin Hamster Syrian Gold Serum

Normal Syrian Hamster Serum

88R-1018 5 ml
EUR 207.6
Description: Normal Syrian Hamster Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Innovative Grade US Origin Hamster Syrian Gold Kidney

IGHMSGKD each
EUR 104
Description: Innovative Grade US Origin Hamster Syrian Gold Kidney

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLAK2E100ML each
EUR 1240
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLAK2E10ML each
EUR 149
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLAK2E25ML each
EUR 349
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLAK2E50ML each
EUR 659
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLAK3E100ML each
EUR 1240
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLAK3E10ML each
EUR 149
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLAK3E25ML each
EUR 349
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLAK3E50ML each
EUR 659
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLALIH100ML each
EUR 1240
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLALIH10ML each
EUR 149
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLALIH25ML each
EUR 349
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLALIH50ML each
EUR 659
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLANAC100ML each
EUR 1240
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLANAC10ML each
EUR 149
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLANAC25ML each
EUR 349
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLANAC50ML each
EUR 659
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLANAE100ML each
EUR 1240
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLANAE10ML each
EUR 149
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLANAE25ML each
EUR 349
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLANAE50ML each
EUR 659
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLANAH100ML each
EUR 1240
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLANAH10ML each
EUR 149
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLANAH25ML each
EUR 349
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Innovative Grade US Origin Hamster Syrian Gold Plasma

IGHMSGPLANAH50ML each
EUR 659
Description: Innovative Grade US Origin Hamster Syrian Gold Plasma

Hamster (Syrian, non-immune) Serum IgM, purified (Syrian)

20003-2-1 0.5 mg
EUR 270

Rat Liver PrimaCell: Hepatocytes

2-82595 1 Kit Ask for price

Hamster (Syrian) IgG Isotype Control

abx405032-025mg 0.25 mg
EUR 460.8

Syrian Hamster IgG Isotype Control

SIGGA-100 100 µg
EUR 688.99

Syrian Hamster IgG Isotype Control

SIGGB-200 200 µg
EUR 566.69

Syrian Hamster IgG Isotype Control

SIGGF-200 200 µg
EUR 654.05

Syrian Hamster IgG Isotype Control

SIGGPE-100 100 µg
EUR 619.1

Syrian Hamster IgG Isotype Control

SIGGPP5.5-100 100 µg
EUR 811.3

Syrian Hamster IgG Isotype Control

SIGGPP5.5-25 25 µg
EUR 426.91

Syrian Hamster IgG Isotype Control

SIGGPU-500 500 µg
EUR 514.27

Syrian Hamster anti-CD28 Antibody

DL99429A-100ul 100 ul
EUR 299
Description: T-cell-specific surface glycoprotein CD28; TP44; CD28

Syrian Hamster anti-CD28 Antibody

DL99429A-50ul 50 ul
EUR 210
Description: T-cell-specific surface glycoprotein CD28; TP44; CD28

Innovative Grade US Origin Hamster Syrian Gold Complement Serum

IGHMSGCSER10ML each
EUR 320
Description: Innovative Grade US Origin Hamster Syrian Gold Complement Serum

Innovative Grade US Origin Hamster Syrian Gold Complement Serum

IGHMSGCSER25ML each
EUR 754
Description: Innovative Grade US Origin Hamster Syrian Gold Complement Serum

Innovative Grade US Origin Hamster Syrian Gold Complement Serum

IGHMSGCSER50ML each
EUR 1420
Description: Innovative Grade US Origin Hamster Syrian Gold Complement Serum

Innovative Grade US Origin Hamster Syrian Gold Complement Serum

IGHMSGCSER5ML each
EUR 200
Description: Innovative Grade US Origin Hamster Syrian Gold Complement Serum

Mouse Liver PrimaCell: Hepatocytes

2-82100 1 Kit Ask for price

Human Liver PrimaCell: Hepatocytes

2-96121 1 Kit Ask for price

Syrian Hamster anti-CD152 Antibody

DL99438A-100ul 100 ul
EUR 299
Description: CD152; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD152

Syrian Hamster anti-CD152 Antibody

DL99438A-50ul 50 ul
EUR 210
Description: CD152; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD152

PE Syrian Hamster IgG Isotype Control

DL22376F-20Tests 20Tests
EUR 158

PE Syrian Hamster IgG Isotype Control

DL22376F-50Tests 50Tests
EUR 233

PE Syrian Hamster IgG Isotype Control

DL22391F-25ug 25μg
EUR 137

PE Syrian Hamster IgG Isotype Control

DL22391F-50ug 50μg
EUR 182

Peroxidase Conjugated Affinity Purified anti-Golden Syrian Hamster IgG [H&L] [Goat]

SH015 250 µg
EUR 125

Hamster (Syrian) IgG Isotype Control (FITC)

abx405033-01mg 0.1 mg
EUR 477.6

FITC Syrian Hamster IgG Isotype Control

DL22375F-20Tests 20Tests
EUR 158

FITC Syrian Hamster IgG Isotype Control

DL22375F-50Tests 50Tests
EUR 233

FITC Syrian Hamster IgG Isotype Control

DL22390F-25ug 25μg
EUR 137

FITC Syrian Hamster IgG Isotype Control

DL22390F-50ug 50μg
EUR 182

AF488 Syrian Hamster IgG Isotype Control

DL22384F-20Tests 20Tests
EUR 185

AF488 Syrian Hamster IgG Isotype Control

DL22384F-50Tests 50Tests
EUR 267.5

AF647 Syrian Hamster IgG Isotype Control

DL22385F-20Tests 20Tests
EUR 185

AF647 Syrian Hamster IgG Isotype Control

DL22385F-50Tests 50Tests
EUR 267.5

AF488 Syrian Hamster IgG Isotype Control

DL22399F-25ug 25μg
EUR 182

AF488 Syrian Hamster IgG Isotype Control

DL22399F-50ug 50μg
EUR 272

AF647 Syrian Hamster IgG Isotype Control

DL22400F-25ug 25μg
EUR 182

AF647 Syrian Hamster IgG Isotype Control

DL22400F-50ug 50μg
EUR 272

Hamster pig hepatocyte growth factor(HGF)ELISA Kit

QY-E90123 96T
EUR 573.6

Biotin Syrian Hamster IgG Isotype Control

DL22389F-25ug 25μg
EUR 137

Biotin Syrian Hamster IgG Isotype Control

DL22389F-50ug 50μg
EUR 182

Goat anti Syrian Hamster IgG (H + L) (HRP)

43R-IG038hrp 1 ml
EUR 510
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (HRP)

Hamster IgG, purified (Syrian, isotype control)

20003-1 1 mg
EUR 169.2

OOIB00014-2ML - Normal Syrian Hamster Serum

OOIB00014-2ML 2ml
EUR 159

OOIB00015-5ML - Normal Syrian Hamster Serum

OOIB00015-5ML 5ml
EUR 219

Goat anti Syrian Hamster IgG (H + L) (FITC)

43R-ID035ft 1 mg
EUR 453.6
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (FITC)

Goat anti Syrian Hamster IgG (H + L) (biotin)

43R-GH001 1 ml
EUR 511.2
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (biotin)

Goat anti Syrian Hamster IgG (H + L) (rhodamine)

43R-IG097rd 1 mg
EUR 411.6
Description: Goat anti Syrian Hamster IgG (H + L) secondary antibody (rhodamine)

DyLight 800 Conjugated Affinity Purified Anti-Golden Syrian & Armenian Hamster IgG [H&L] [Goat]

SD013 100 µg
EUR 200

Syrian Hamster IgG Isotype Control(APC Conjugated)

DL22377F-20Tests 20 Tests
EUR 120

ERY at concentrations up to 60 pM non-toxic to 6 days of incubation. Use of ERY to selectively inhibit CYP3A in hepatocytes, long-term high-functioning as HepatoPac can be a valuable strategy to evaluate the contribution