Cleavage of cyclic AMP-responsive element-binding protein H aggravates myocardial hypoxia reperfusion injury in a hepatocyte-myocardial cell co-culture system.

This study aims to determine whether the proinflammatory cytokines have effects on myocardial cells (MC) and hepatocytes during myocardial ischemia to induce cyclic element-binding protein H (CREBH) cleavage AMP-responsive, enabling the acute phase response in the liver, and cause injury MCs.In superimposed on this research, transwell co-culture system hepatocyte-MC is used to investigate the relationship between the injury of hypoxia / reperfusion myocardial and CREBH cleavage.

MC and neonatal rat hepatocytes derived from the ventricles and heart Sprague Dawley rats each. MC inoculated into the lower chamber of the transwell chamber for 12 hours under hypoxia. The level of endoplasmic reticulum stress protein glucose-regulated protein 78 in MC, CREBH in hepatocytes, inflammatory factors (tumor necrosis factor-α and interleukin-6) levels, and cell viability is evaluated.

CREBH influence knockdown was also studied using short hairpin RNA specifically CREBH (Ad-CREBHi) .We found that proinflammatory cytokines affect MC and hepatocytes during myocardial ischemia to induce cleavage CREBH, activate the acute phase response in the liver, and cause injury superimposed on MCs. Expression of CREBH exacerbate the myocardial injury during myocardial ischemia.

Development co-culture model of mouse primary hepatocytes and splenocytes to evaluate the genotoxicity of xenobiotics using medium-throughput Comet assay.

To date, only a limited number of toxicology studies have focused on the establishment and validation of in vitro genotoxicity screening system using primary hepatocytes, and the results of these studies have been inconsistent. Therefore, the aim of this study was to develop a model of effective co-culture of rat primary hepatocytes and splenocytes derived for screening chemicals for medium-throughput genotoxicity using the Comet assay.

Cleavage of cyclic AMP-responsive element-binding protein H aggravates myocardial hypoxia reperfusion injury in a hepatocyte-myocardial cell co-culture system.
Cleavage of cyclic AMP-responsive element-binding protein H aggravates myocardial hypoxia reperfusion injury in a hepatocyte-myocardial cell co-culture system.

Cocultured model is built and verified using known genotoxic and non-genotoxic compounds as positive and negative controls, respectively. Cytotoxicity was measured using Cell Counting Kit-8 and lactate dehydrogenase method. DNA damage was detected using both alkaline and formamidopyrimidine glikosilase DNA test (FPG) Comet. Compared with controls, DNA strand breaks and FPG-sensitive sites showed a significant concentration-dependent increase in group-treated genotoxic agents.

In contrast, DNA damage remains unchanged in groups of non-genotoxic-agent-treated. In addition, various types of DNA lesions lead to genotoxic agents depending on different times.

Liver Dissociation System 4 (Hepatocytes, rat), Rat

4-20304 ea Ask for price

Mouse Liver PrimaCell: Hepatocytes

2-82100 1 Kit Ask for price

Human Liver PrimaCell: Hepatocytes

2-96121 1 Kit Ask for price

Human Hepatocytes - Alcohol Addicted

HC4230AA 500,000 Cells
EUR 957

Human Hepatocytes - Opioid Addicted

HC4230OP 500000
EUR 957

Immortalized Human Hepatocytes - hTERT

T0058 1x106 cells / 1.0 ml Ask for price

Immortalized Human Hepatocytes - Ras

T0059 1x106 cells / 1.0 ml Ask for price

Immortalized Monkey Hepatocytes-SV40

T0073 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Hepatocytes - SV40

T0101 1x106 cells / 1.0 ml Ask for price

Neosartorya fischer One-Step PCR kit

Oneq-A570-100D 100T
EUR 866

Neosartorya fischer One-Step PCR kit

Oneq-A570-150D 150T
EUR 981

Neosartorya fischer One-Step PCR kit

Oneq-A570-50D 50T
EUR 718

Rat Liver PrimaCell: Normal Hepatocytes Growth Medium

9-25095 5 x 100 ml Ask for price

Rat Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80322 1 x 100 ml Ask for price

Immortalized Mouse Hepatocytes-Conditionally (ImHep)

T0099 1x106 cells / 1.0 ml Ask for price

Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)

RAT-5 1
EUR 1138

Liver Dissociation System 1 (Hepatocytes), Mouse

4-20301 ea Ask for price

Immortalized Hamster Hepatocytes-SV40 T+t

T0071 1x106 cells / 1.0 ml Ask for price

Immortalized Monkey Hepatocytes-SV40 T+t

T0074 1x106 cells / 1.0 ml Ask for price

TnI (Primary) Antibody

abx018056-100ug 100 ug
EUR 342

Primary Human Neurons

HNC001 300,000 Cyroperserved Cells
EUR 1223

Liver Dissociation System 6 (Hepatocytes,Nonparenchymal), Mouse and Rat

4-20306 ea Ask for price

Rat Primary hepatic carcinoma ELISA kit

E02P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Primary hepatic carcinoma ELISA kit

E02P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Primary hepatic carcinoma ELISA kit

E02P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Liver PrimaCell: Normal Hepatocytes Growth Medium

9-32100 5 x 100 ml Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Medium

9-46121 5 x 100 ml Ask for price

Mouse Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80306 1 x 100 ml Ask for price

Human Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80314 1 x 100 ml Ask for price

Liver Dissociation System 3 (Hepatocytes, Kupffer, Parenchymal), Mouse and Rat

4-20303 ea Ask for price

Primary Antibody Dropper Vial

PAV015 1 ea.
EUR 53

DB Primary Antibody diluent

DB-D-125 125 ml
EUR 150

DB Primary Antibody diluent

DB-D-250 250 ml
EUR 245

DB Primary Antibody diluent

DBD-125 125 ml
EUR 150

DB Primary Antibody diluent

DBD-250 250 ml
EUR 245

Antigen-Antibody Pen For Rat Primary antibodies

PEN-R10 1
EUR 202

Rat Aoc3/ Membrane primary amine oxidase ELISA Kit

E0075Ra 1 Kit
EUR 571

Rat Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-82522 1 Kit Ask for price

ELISA kit for Rat Membrane primary amine oxidase

EK2706 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Membrane primary amine oxidase in samples from serum, plasma, tissue homogenates and other biological fluids.

Rat Aoc3(Membrane primary amine oxidase) ELISA Kit

ER0434 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.469 ng/ml

Rat Membrane primary amine oxidase(AOC3) ELISA kit

CSB-EL001855RA-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Rat Membrane primary amine oxidase (AOC3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Membrane primary amine oxidase(AOC3) ELISA kit

1-CSB-EL001855RA
  • EUR 703.00
  • EUR 4843.00
  • EUR 2570.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Membrane primary amine oxidase(AOC3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Myeloid differentiation primary response protein MyD88, Myd8

ELI-05538r 96 Tests
EUR 886

Primary Antibody Diluent (Phosphate, Green)

APG010 10 L
EUR 671

Primary Antibody Diluent (Phosphate, Green)

APG125 125 ml
EUR 74

Primary Antibody Diluent (Phosphate, Green)

APG500 500 ml
EUR 101

Primary Antibody Diluent (Phosphate, Green)

APG999 1000 ml
EUR 124

Primary Antibody Diluent (Tris, Green)

ATG-20000 20 L
EUR 1177

Primary Antibody Diluent (Tris, Green)

ATG125 125 ml
EUR 74

Primary Antibody Diluent (Tris, Green)

ATG500 500 ml
EUR 101

Primary Antibody Diluent (Tris, Green)

ATG999 1000 ml
EUR 124

Western blot Kit for Rat Primary Antibodies, Chemilum. Substrate

80203-Rt 1 kit
EUR 651

Rat Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-26095 1 Set Ask for price

Membrane Primary Amine Oxidase (AOC3) Antibody

20-abx110946
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Myeloid Differentiation Primary Response 88 Antibody

20-abx137458
  • EUR 704.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 20 ug
  • 5 ug

Western Blot Primary Antibody Diluent Buffer

abx090689-100ml 100 ml
EUR 182

Membrane Primary Amine Oxidase (AOC3) Antibody

20-abx001627
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Membrane Primary Amine Oxidase (AOC3) Antibody

abx033988-400ul 400 ul
EUR 523

Membrane Primary Amine Oxidase (AOC3) Antibody

abx033988-80l 80 µl
EUR 286

Myeloid Differentiation Primary Response 88 Protein

20-abx261025
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Membrane Primary Amine Oxidase (AOC3) Antibody

20-abx333876
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Pig Primary hepatic carcinoma ELISA kit

E07P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Primary hepatic carcinoma ELISA kit

E07P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Primary hepatic carcinoma ELISA kit

E07P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit

E08P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit

E08P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit

E08P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit

E04P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit

E04P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit

E04P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit

E06P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit

E06P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit

E06P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit

E03P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit

E03P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit

E03P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Primary hepatic carcinoma ELISA kit

E01P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Primary hepatic carcinoma ELISA kit

E01P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Primary hepatic carcinoma ELISA kit

E01P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit

E09P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit

E09P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit

E09P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myd88/ Myeloid differentiation primary response protein MyD88 ELISA Kit

E0652Ra 1 Kit
EUR 571

Rat Breast PrimaCell: Normal Mammary Epithelial Primary Cells Growth Medium

9-25022 5 x 100 ml Ask for price

Rat Breast Tissue Preparation Buffer: Normal Mammary Epithelial Primary Cells

9-80229 1 x 100 ml Ask for price

Aoc3 ELISA Kit| Rat Membrane primary amine oxidase ELISA Kit

EF017277 96 Tests
EUR 689

Rat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit

E02M0411-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit

E02M0411-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit

E02M0411-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human primary hepatic carcinoma(PHC)ELISA Kit

GA-E1689HM-48T 48T
EUR 289

Human primary hepatic carcinoma(PHC)ELISA Kit

GA-E1689HM-96T 96T
EUR 466

Human primary hepatic carcinoma,PHC ELISA Kit

201-12-1673 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Our results show that the tail DNA% showed both strands of DNA break and FPG-sensitive sites may be an effective marker for predicting chemical-induced DNA damage and oxidative DNA damage using a model cocultured hepatocytes and splenocytes. Collectively, these findings provide reliable experimental data for the establishment of in vitro genotoxicity screening methods.