Cleavage of cyclic AMP-responsive element-binding protein H aggravates myocardial hypoxia reperfusion injury in a hepatocyte-myocardial cell co-culture system.
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Cleavage of cyclic AMP-responsive element-binding protein H aggravates myocardial hypoxia reperfusion injury in a hepatocyte-myocardial cell co-culture system.
This study aims to determine whether the proinflammatory cytokines have effects on myocardial cells (MC) and hepatocytes during myocardial ischemia to induce cyclic element-binding protein H (CREBH) cleavage AMP-responsive, enabling the acute phase response in the liver, and cause injury MCs.In superimposed on this research, transwell co-culture system hepatocyte-MC is used to investigate the relationship between the injury of hypoxia / reperfusion myocardial and CREBH cleavage.
MC and neonatal rat hepatocytes derived from the ventricles and heart Sprague Dawley rats each. MC inoculated into the lower chamber of the transwell chamber for 12 hours under hypoxia. The level of endoplasmic reticulum stress protein glucose-regulated protein 78 in MC, CREBH in hepatocytes, inflammatory factors (tumor necrosis factor-α and interleukin-6) levels, and cell viability is evaluated.
CREBH influence knockdown was also studied using short hairpin RNA specifically CREBH (Ad-CREBHi) .We found that proinflammatory cytokines affect MC and hepatocytes during myocardial ischemia to induce cleavage CREBH, activate the acute phase response in the liver, and cause injury superimposed on MCs. Expression of CREBH exacerbate the myocardial injury during myocardial ischemia.
Development co-culture model of mouse primary hepatocytes and splenocytes to evaluate the genotoxicity of xenobiotics using medium-throughput Comet assay.
To date, only a limited number of toxicology studies have focused on the establishment and validation of in vitro genotoxicity screening system using primary hepatocytes, and the results of these studies have been inconsistent. Therefore, the aim of this study was to develop a model of effective co-culture of rat primary hepatocytes and splenocytes derived for screening chemicals for medium-throughput genotoxicity using the Comet assay.
Cleavage of cyclic AMP-responsive element-binding protein H aggravates myocardial hypoxia reperfusion injury in a hepatocyte-myocardial cell co-culture system.
Cocultured model is built and verified using known genotoxic and non-genotoxic compounds as positive and negative controls, respectively. Cytotoxicity was measured using Cell Counting Kit-8 and lactate dehydrogenase method. DNA damage was detected using both alkaline and formamidopyrimidine glikosilase DNA test (FPG) Comet. Compared with controls, DNA strand breaks and FPG-sensitive sites showed a significant concentration-dependent increase in group-treated genotoxic agents.
In contrast, DNA damage remains unchanged in groups of non-genotoxic-agent-treated. In addition, various types of DNA lesions lead to genotoxic agents depending on different times.
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Liver PrimaCell: Normal Hepatocytes Growth Medium
Description: Quantitativesandwich ELISA kit for measuring Rat Membrane primary amine oxidase (AOC3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Rat Membrane primary amine oxidase(AOC3) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Rat Membrane primary amine oxidase(AOC3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Rat Aoc3/ Membrane primary amine oxidase ELISA Kit
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Membrane primary amine oxidase in samples from serum, plasma, tissue homogenates and other biological fluids.
Rat Myeloid differentiation primary response protein MyD88, Myd8
Description: This product includes 0.5 ml of 10 x PPA dye, 5.5 ml of Assay buffer, 5 ml of reagent A and 0.05 ml of 1 mM methylamine-HCl. It is for measurement of 100 samples using 96-well plates. Cuvettes may also be used for measurements.
Western blot Kit for Rat Primary Antibodies, Chemilum. Substrate
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human 293, transformed primary embryonal kidney lysate
Description: Quantitativesandwich ELISA kit for measuring Human Membrane primary amine oxidase (AOC3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Human Membrane primary amine oxidase(AOC3) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Human Membrane primary amine oxidase(AOC3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Mouse Membrane primary amine oxidase (AOC3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse Membrane primary amine oxidase(AOC3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Membrane primary amine oxidase in samples from serum, plasma, tissue homogenates and other biological fluids.
ELISA kit for Human Membrane primary amine oxidase
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Membrane primary amine oxidase in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: MYD88 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 345 amino acids (1-309 a.a) and having a molecular mass of 38.7kDa.;MYD88 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Ovary primary carcinoma with matched metastatic carcinoma tissue array
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Goat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
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Rabbit Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human MYD88(Myeloid differentiation primary response protein MyD88) ELISA Kit
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Monkey Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human MYD88/ Myeloid differentiation primary response protein MyD88 ELISA Kit
Description: A competitive ELISA for quantitative measurement of Canine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
ELISA kit for Human Myeloid differentiation primary response protein MyD88
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Myeloid differentiation primary response protein MyD88 in samples from serum, plasma, tissue homogenates and other biological fluids.
ELISA kit for Mouse Myeloid differentiation primary response protein MyD88
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Myeloid differentiation primary response protein MyD88 in samples from serum, plasma, tissue homogenates and other biological fluids.
Human Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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Our results show that the tail DNA% showed both strands of DNA break and FPG-sensitive sites may be an effective marker for predicting chemical-induced DNA damage and oxidative DNA damage using a model cocultured hepatocytes and splenocytes. Collectively, these findings provide reliable experimental data for the establishment of in vitro genotoxicity screening methods.