Decellularized liver matrix-modified chitosan fibrous scaffold as a substrate for C3A hepatocyte culture.
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Decellularized liver matrix-modified chitosan fibrous scaffold as a substrate for C3A hepatocyte culture.
A bioreactor filled with functional hepatocytes is an important part of the bio-artificial liver device. However, it is a difficult task to maintain a sufficient number of cells and hepatocellular function is active. In this work, we developed a promising scaffold for hepatocyte culture by coating the pork liver extracellular matrix (ECM) in chitosan (CTS) fabric.
Porcine hearts were decellularized using 1% Triton X-100. ECM dissolved CTS heart was immobilized on the fiber surface through cross linking of ECM and CTS with 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS). Then scaffold characterized by Fourier transformed infrared spectroscopy attenuated total reflection mode (ATR-FTIR), X-photoelectron spectroscopy (XPS) and water contact angle measurements.
Efficacy scaffold modified to maintain hepatocyte C3A adhesion, proliferation, and functionality in vitro bioactivity detected. FTIR spectra and XPS show their ECM CTS coating on the fabric surface. Covalent coating significantly increases the efficiency of binding between ECM and CTS fabric, compared with a coating by physical absorption.
Furthermore, the C3A hepatocytes cultured on the scaffold showed an increase bioactivity coated cells and liver-specific functions, such as albumin secretion and synthesis of urea, compared to those cultivated untreated scaffold (p <0.05). As a carrier of culture hepatocytes promising, ECM coated fabric CTS can be applied in biological artificial liver reactors.
Decellularized liver matrix-modified chitosan fibrous scaffold as a substrate for C3A hepatocyte culture.
a comparative study between the lactose-silk fibroin and extracellular matrix conjugate as a substrate for the culture of human induced pluripotent stem cell-derived hepatocytes.
Human induced pluripotent stem cells (hiPSC) -derived hepatocytes a cell source attractive alternative to primary human hepatocytes for studies network regeneration.This gift an app from lactose-silk conjugate (Lac-CY-SF) bearing β-galactose residue as substrate hiPSC derived hepatocytes for culture.
Comparison hiPSC derived hepatocytes were cultured on three different substrates; Lac-CY-SF conjugates, Matrigel and type I collagen is performed.Cell morphology, viability, maturation and function of albumin secretion was assessed by phase contrast microscopy, tetrazolium based colorimetric assay, immunofluorescence staining and characteristics of the enzyme-linked immunosorbent assay.
Morphological of cell- cells were cultured on a conjugate resemble those in Matrigel throughout the 6-day culture period. The number of viable cells cultivated in the conjugate is comparable to that in Matrigel at day 2 and 6. The protein expression of hepatocyte markers of adulthood, asialoglycoprotein receptor 1 and albumin, the cells are cultured on a conjugate resembles that in cells cultured in collagen on days 2 and 6.
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Hepatocyte Growth Factor (HGF)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Quantitative sandwich ELISA kit for measuring Human hepatocyte growth factor, HGF in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative sandwich ELISA kit for measuring Human hepatocyte growth factor, HGF in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Human Hepatocyte Growth Factor (HGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Hepatocyte Growth Factor (HGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Hepatocyte growth factor in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Hepatocyte Growth Factor (HGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Recombinant HGF alpha is a non-glycosylated disulfide-linked protein consisting of the alpha chain (463) amino acid residues, and migrates as an approximately 54 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Hepatocyte Growth Factor alpha chain was expressed in E. coli.
Description: Recombinant HGF alpha is a non-glycosylated disulfide-linked protein consisting of the alpha chain (463) amino acid residues, and migrates as an approximately 54 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Hepatocyte Growth Factor alpha chain was expressed in E. coli.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 1 β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 1 β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 1 β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 6 β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 6 β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 6 β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA kit for detection of Hepatocyte Growth Factor from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Human Hepatocyte Growth Factor Like (MST1) ELISA Kit
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 1 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 1 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 1 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 4 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 4 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 4 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 6 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 6 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 6 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in Tissue homogenates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in Tissue homogenates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in Tissue homogenates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in Tissue homogenates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) CLIA Kit
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b)Tissue homogenates and other biological fluids
Human Hepatocyte Growth Factor (HGF) Protein (Active)
Albumin function of secretion per cell cultured on a conjugate higher than in collagen and comparable to that in the limited Matrigel.These results showed that Lac-CY-SF conjugates may be useful as Matrigel and collagen for cultivation hiPSC derived hepatocytes.
