Decellularized liver matrix-modified chitosan fibrous scaffold as a substrate for C3A hepatocyte culture.

A bioreactor filled with functional hepatocytes is an important part of the bio-artificial liver device. However, it is a difficult task to maintain a sufficient number of cells and hepatocellular function is active. In this work, we developed a promising scaffold for hepatocyte culture by coating the pork liver extracellular matrix (ECM) in chitosan (CTS) fabric.

Porcine hearts were decellularized using 1% Triton X-100. ECM dissolved CTS heart was immobilized on the fiber surface through cross linking of ECM and CTS with 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS). Then scaffold characterized by Fourier transformed infrared spectroscopy attenuated total reflection mode (ATR-FTIR), X-photoelectron spectroscopy (XPS) and water contact angle measurements.

Efficacy scaffold modified to maintain hepatocyte C3A adhesion, proliferation, and functionality in vitro bioactivity detected. FTIR spectra and XPS show their ECM CTS coating on the fabric surface. Covalent coating significantly increases the efficiency of binding between ECM and CTS fabric, compared with a coating by physical absorption.

Furthermore, the C3A hepatocytes cultured on the scaffold showed an increase bioactivity coated cells and liver-specific functions, such as albumin secretion and synthesis of urea, compared to those cultivated untreated scaffold (p <0.05). As a carrier of culture hepatocytes promising, ECM coated fabric CTS can be applied in biological artificial liver reactors.

Decellularized liver matrix-modified chitosan fibrous scaffold as a substrate for C3A hepatocyte culture.
Decellularized liver matrix-modified chitosan fibrous scaffold as a substrate for C3A hepatocyte culture.

a comparative study between the lactose-silk fibroin and extracellular matrix conjugate as a substrate for the culture of human induced pluripotent stem cell-derived hepatocytes.

Human induced pluripotent stem cells (hiPSC) -derived hepatocytes a cell source attractive alternative to primary human hepatocytes for studies network regeneration.This gift an app from lactose-silk conjugate (Lac-CY-SF) bearing β-galactose residue as substrate hiPSC derived hepatocytes for culture.

Comparison hiPSC derived hepatocytes were cultured on three different substrates; Lac-CY-SF conjugates, Matrigel and type I collagen is performed.Cell morphology, viability, maturation and function of albumin secretion was assessed by phase contrast microscopy, tetrazolium based colorimetric assay, immunofluorescence staining and characteristics of the enzyme-linked immunosorbent assay.

Morphological of cell- cells were cultured on a conjugate resemble those in Matrigel throughout the 6-day culture period. The number of viable cells cultivated in the conjugate is comparable to that in Matrigel at day 2 and 6. The protein expression of hepatocyte markers of adulthood, asialoglycoprotein receptor 1 and albumin, the cells are cultured on a conjugate resembles that in cells cultured in collagen on days 2 and 6.

Mouse Liver PrimaCell: Hepatocytes

2-82100 1 Kit Ask for price

Rat Liver PrimaCell: Hepatocytes

2-82595 1 Kit Ask for price

Immortalized Monkey Hepatocytes-SV40

T0073 1x106 cells / 1.0 ml Ask for price

Immortalized Rat Hepatocytes - SV40

T0078 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Hepatocytes - SV40

T0101 1x106 cells / 1.0 ml Ask for price

Primary Human Neurons

HNC001 300,000 Cyroperserved Cells
EUR 1223

Immortalized Mouse Hepatocytes-Conditionally (ImHep)

T0099 1x106 cells / 1.0 ml Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Medium

9-46121 5 x 100 ml Ask for price

Human Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80314 1 x 100 ml Ask for price

Liver Dissociation System 1 (Hepatocytes), Mouse

4-20301 ea Ask for price

Immortalized Hamster Hepatocytes-SV40 T+t

T0071 1x106 cells / 1.0 ml Ask for price

Immortalized Monkey Hepatocytes-SV40 T+t

T0074 1x106 cells / 1.0 ml Ask for price

TnI (Primary) Antibody

abx018056-100ug 100 ug
EUR 342

Liver Dissociation System 4 (Hepatocytes, rat), Rat

4-20304 ea Ask for price

Rat Liver PrimaCell: Normal Hepatocytes Growth Medium

9-25095 5 x 100 ml Ask for price

Mouse Liver PrimaCell: Normal Hepatocytes Growth Medium

9-32100 5 x 100 ml Ask for price

Mouse Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80306 1 x 100 ml Ask for price

Rat Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80322 1 x 100 ml Ask for price

Primary Antibody Dropper Vial

PAV015 1 ea.
EUR 53

DB Primary Antibody diluent

DB-D-125 125 ml
EUR 150

DB Primary Antibody diluent

DB-D-250 250 ml
EUR 245

DB Primary Antibody diluent

DBD-125 125 ml
EUR 150

DB Primary Antibody diluent

DBD-250 250 ml
EUR 245

Human Primary hepatic carcinoma ELISA kit

E01P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Primary hepatic carcinoma ELISA kit

E01P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Primary hepatic carcinoma ELISA kit

E01P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human primary hepatic carcinoma(PHC)ELISA Kit

GA-E1689HM-48T 48T
EUR 289

Human primary hepatic carcinoma(PHC)ELISA Kit

GA-E1689HM-96T 96T
EUR 466

Human primary hepatic carcinoma,PHC ELISA Kit

201-12-1673 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Antigen-Antibody Pen For Human Primary antibodies

PEN-H7 1
EUR 202

Human primary hepatic carcinoma(PHC)ELISA Kit

QY-E03406 96T
EUR 361

Human 293, transformed primary embryonal kidney lysate

HCL-1210 100ug
EUR 213

Primary Antibody Diluent (Phosphate, Green)

APG010 10 L
EUR 671

Primary Antibody Diluent (Phosphate, Green)

APG125 125 ml
EUR 74

Primary Antibody Diluent (Phosphate, Green)

APG500 500 ml
EUR 101

Primary Antibody Diluent (Phosphate, Green)

APG999 1000 ml
EUR 124

Primary Antibody Diluent (Tris, Green)

ATG-20000 20 L
EUR 1177

Primary Antibody Diluent (Tris, Green)

ATG125 125 ml
EUR 74

Primary Antibody Diluent (Tris, Green)

ATG500 500 ml
EUR 101

Primary Antibody Diluent (Tris, Green)

ATG999 1000 ml
EUR 124

Liver Dissociation System 6 (Hepatocytes,Nonparenchymal), Mouse and Rat

4-20306 ea Ask for price

Human AOC3/ Membrane primary amine oxidase ELISA Kit

E2737Hu 1 Kit
EUR 537

Human Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-96023 1 Kit Ask for price

ELISA kit for Human Membrane primary amine oxidase

EK2705 96 tests
EUR 452
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Membrane primary amine oxidase in samples from serum, plasma, tissue homogenates and other biological fluids.

Human Myeloid differentiation primary response protein MyD88 (MYD88)

1-CSB-EP859945HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Myeloid differentiation primary response protein MyD88(MYD88) expressed in E.coli

Human Membrane primary amine oxidase(AOC3) ELISA kit

CSB-EL001855HU-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human Membrane primary amine oxidase (AOC3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Membrane primary amine oxidase(AOC3) ELISA kit

1-CSB-EL001855HU
  • EUR 703.00
  • EUR 4843.00
  • EUR 2570.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Membrane primary amine oxidase(AOC3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human AOC3(Membrane primary amine oxidase ) ELISA Kit

EH0577 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml

Human Membrane primary amine oxidase, AOC3 ELISA KIT

ELI-03529h 96 Tests
EUR 824

Human Myeloid differentiation primary response protein MyD88, MY

ELI-05535h 96 Tests
EUR 824

Membrane Primary Amine Oxidase (AOC3) Antibody

20-abx110946
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Myeloid Differentiation Primary Response 88 Antibody

20-abx137458
  • EUR 704.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 20 ug
  • 5 ug

Western Blot Primary Antibody Diluent Buffer

abx090689-100ml 100 ml
EUR 182

Membrane Primary Amine Oxidase (AOC3) Antibody

20-abx001627
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Membrane Primary Amine Oxidase (AOC3) Antibody

abx033988-400ul 400 ul
EUR 523

Membrane Primary Amine Oxidase (AOC3) Antibody

abx033988-80l 80 µl
EUR 286

Myeloid Differentiation Primary Response 88 Protein

20-abx261025
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Membrane Primary Amine Oxidase (AOC3) Antibody

20-abx333876
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Pig Primary hepatic carcinoma ELISA kit

E07P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Primary hepatic carcinoma ELISA kit

E07P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Primary hepatic carcinoma ELISA kit

E07P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit

E08P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit

E08P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit

E08P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit

E04P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit

E04P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit

E04P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit

E06P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit

E06P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit

E06P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit

E03P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit

E03P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit

E03P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Primary hepatic carcinoma ELISA kit

E02P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Primary hepatic carcinoma ELISA kit

E02P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Primary hepatic carcinoma ELISA kit

E02P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit

E09P0656-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit

E09P0656-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit

E09P0656-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Liver Dissociation System 3 (Hepatocytes, Kupffer, Parenchymal), Mouse and Rat

4-20303 ea Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-47121 1 Set Ask for price

MYD88 Myeloid Differentiation Primary Response 88 Human Recombinant Protein

PROTQ99836 Regular: 20ug
EUR 317
Description: MYD88 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 345 amino acids (1-309 a.a) and having a molecular mass of 38.7kDa.;MYD88 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Western blot Kit for Human Primary Antibodies, Chemilum. Substrate

80207-Hu 1 kit
EUR 651

Recombinant Human Membrane Primary Amine Oxidase/AOC3(C-6His)

C927-10ug 10ug
EUR 232
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0.

Recombinant Human Membrane Primary Amine Oxidase/AOC3(C-6His)

C927-1mg 1mg
EUR 3602
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0.

Recombinant Human Membrane Primary Amine Oxidase/AOC3(C-6His)

C927-500ug 500ug
EUR 2536
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0.

Recombinant Human Membrane Primary Amine Oxidase/AOC3(C-6His)

C927-50ug 50ug
EUR 598
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0.

Human Primary ciliary dyskinesia protein 1, PCDP1 ELISA KIT

ELI-45084h 96 Tests
EUR 824

Membrane Primary Amine Oxidase (AOC3) Antibody (HRP)

20-abx336489
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Membrane Primary Amine Oxidase (AOC3) Antibody (FITC)

20-abx336490
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Membrane Primary Amine Oxidase (AOC3) Antibody (Biotin)

20-abx336491
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Antigen-Antibody Pen For Chicken Primary antibodies

PEN-C5 1
EUR 202

Albumin function of secretion per cell cultured on a conjugate higher than in collagen and comparable to that in the limited Matrigel.These results showed that Lac-CY-SF conjugates may be useful as Matrigel and collagen for cultivation hiPSC derived hepatocytes.