A simple method for isolation and culture of primary hepatocytes from Salvelinus leucomaenis (White-spotted Charr)
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A simple method for isolation and culture of primary hepatocytes from Salvelinus leucomaenis (White-spotted Charr)
White-spotted charr (Salvelinus leucomaenis, S. I.) is adjusted anadromous fish cold water, distributed in the Far East. We have previously reported the complete mitochondrial DNA sequence of white-spotted characters (l. S. imbrius and S. l. Pluvius) in Japan.
In general, fish hepatocytes useful for the study of cellular and biochemical fish. In this study, we isolated hepatocytes from the liver and white speckled charr and use basic methods, such as digestive enzymes and low centrifugation, to analyze the molecular mechanisms involved in specific cellular responses. isolated hepatocytes can be cultured at 5-20 ° C but not 37 ° C. The morphology of hepatocytes has been altered in a manner dependent on the temperature.
The properties of hepatocytes similar to the fish live. In addition, the rate of proliferation and isolated hepatocytes damage depends on the concentration of fetal bovine serum in the culture medium. Taken together, these studies show that this simple method for the isolation and culture of hepatocytes from white speckled charr may be useful for the study of cellular biochemistry and others.
A simple method for isolation and culture of primary hepatocytes from Salvelinus leucomaenis (White-spotted Charr)
A 3D Cell Culture Model Identifies the Wnt / β-Catenin Mediated Inhibition of p53 as Critical Steps for Human Hepatocyte Regeneration
The liver is the organ that is regenerative. While the mature hepatocytes in a state of homeostasis largely silent, after injury, they quickly enter the cell cycle to restore damaged tissue.
In rodents, various models of injury has provided important insights into the fundamentals of molecular regulate hepatocyte proliferation activation silent. However, little is known about the molecular mechanisms of regeneration of human hepatocytes and experimental methods to expand primary human hepatocytes (PHH). Here, the 3D model spheroid of PHH established for the regeneration of hepatocytes and integrative studies of time-lapse multi-omics analysis showed that after the isolation of indigenous people acquire PHH liver regenerative phenotype, as seen in vivo in partial hepatectomy.
However, the proliferation is limited. By analyzing global patterns promoter activity, it is estimated that the activation of Wnt / β-catenin signaling and inhibition of p53 is an important factor that is required for the proliferation of human hepatocytes. functional validation revealed that the activation of Wnt signaling through external cues alone is sufficient to inhibit p53 and proliferation-inducing aging its target PAI1 (SERPINE1) and drive the proliferation of> 50% of all PHH.
Mouse Liver Tissue Preparation Buffer: Normal Hepatocytes
Description: The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism, biotransformation of xenobiotics, and synthesis of plasma proteins under physiological and pathophysiological conditions. Primary human hepatocyte culture is an important experimental model for the study of liver specific function. Propagation of human hepatocytes is critical for gene therapy, cell transplantation and clinical liver support systems. In appropriate culture condition, cultured human hepatocytes proliferate and maintain differentiated hepatocyte function such as the synthesis of serum proteins. HH from Gentaur Research Laboratories are isolated from human liver. HH are cryopreserved immediately after purification and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume. HH are characterized by immunofluorescent method with antibodies to albumin, cytokeratin-18 and vimentin. HH are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HH is not recommended for expanding or long term cultures since the cells do not proliferate in culture.
Description: The cell line was prepared by immortalizing hepatocytes from a 12 year-old Caucasian female donor with the SV40 large T antigen. The donor tested negative for CMV, HIV, HBV, and HCV.
Description: Freshly isolated human hepatocytes represent a living biological system in which to measure metabolism, induction, toxicity, and transporter studies.
Description: Human Pooled Primary Hepatocytes (Heps) provide an ideal culture model for drug testing and drug development.Heps 20 Human Pooled Primary Hepatocytes contain cryopreserved hepatocytes pooled from 20 different donors and have undergone only one freeze cycle. Pooled hepatocytes provide the genetic diversity necessary for superior reliability and predictability of the metabolic characteristics of a test compound relative to a representative population. Pooled hepatocytes increase experimental control and reduce costs by eliminating the need to conduct redundant experiments necessary to obtain population-based data. Each lot has undergone a single freeze cycle, assuring maximum hepatocyte viability and consistency from donor to donor within the pool.Cell Features:Each donor's hepatocytes have been cryopreserved ONCE immediately after isolation and purification.Pools of 10 or 20 donors are available. Each donor is represented by a statistically significant number of cells relative to the pool.There is extensive quality control providing characterization of viability and enzyme activity from both individual donors and the total pool.Gentaur guarantees performance and quality.Donor information is available.
Description: Human Pooled Primary Hepatocytes (HP2 Heps) provide an ideal culture model for drug testing and drug development. HP2 10 Human Pooled Primary Hepatocytes contain cryopreserved hepatocytes pooled from 10 different donors and have undergone only one freeze cycle. Pooled hepatocytes provide the genetic diversity necessary for superior reliability and predictability of the metabolic characteristics of a test compound relative to a representative population. Pooled hepatocytes increase experimental control and reduce costs by eliminating the need to conduct redundant experiments necessary to obtain population-based data. Each lot has undergone a single freeze cycle, assuring maximum hepatocyte viability and consistency from donor to donor within the pool.Cell Features:Each donor's hepatocytes have been cryopreserved ONCE immediately after isolation and purification.Pools of 10 or 20 donors are available. Each donor is represented by a statistically significant number of cells relative to the pool.There is extensive quality control providing characterization of viability and enzyme activity from both individual donors and the total pool.Gentaur guarantees performance and quality.Donor information is available.
Description: Gentaur's hepatocytes for suspension assays (HP1 Heps-s - single donor, male) provide an economic source of characterized human hepatocytes for use in short-term suspension assays to evaluate the metabolism of drug candidates. Gentaur's hepatocytes are a functional, convenient and economical solution for your drug testing and basic research needs. Each lot is provided with extensive characterization of metabolic functionality to ensure that you receive an ideal model for drug testing, drug development or other metabolic studies. Gentaur's human hepatocytes eliminate the need and expense of obtaining liver tissue, and conducting multiple cell isolations; and Gentaur also provides hepatocyte-tested culture reagents and instructions necessary for thawing and culturing your hepatocytes.Cell Features:Suitable for induction assays or metabolic characterization assays in plated or suspension cultures. Plated cultures are excellent for long-term intrinsic clearance assays.Hepatocytes are cryopreserved ONCE immediately after isolation and purification.Characterization of viability and enzyme activity, including fold-induction of CYP1A2, CYP2B6 and CYP3A4 for HP1 Heps-i.Gentaur guarantees performance and quality.Donor demographics and medical information are available.
Description: Gentaur's hepatocytes for suspension assays (HP1 Heps-s, single donor, female) provide an economic source of characterized human hepatocytes for use in short-term suspension assays to evaluate the metabolism of drug candidates. Gentaur's hepatocytes are a functional, convenient and economical solution for your drug testing and basic research needs. Each lot is provided with extensive characterization of metabolic functionality to ensure that you receive an ideal model for drug testing, drug development or other metabolic studies. Gentaur's human hepatocytes eliminate the need and expense of obtaining liver tissue, and conducting multiple cell isolations; and Gentaur also provides hepatocyte-tested culture reagents and instructions necessary for thawing and culturing your hepatocytes.Cell Features:Suitable for induction assays or metabolic characterization assays in plated or suspension cultures. Plated cultures are excellent for long-term intrinsic clearance assays.Hepatocytes are cryopreserved ONCE immediately after isolation and purification.Characterization of viability and enzyme activity, including fold-induction of CYP1A2, CYP2B6 and CYP3A4 for HP1 Heps-i.Gentaur guarantees performance and quality.Donor demographics and medical information are available.
Description: Our High Quality Mouse Hepatocytes have been isolated from mouse livers after liver perfusion and low-speed differential centrifugation. The hepatocytes are cryoperserved immediately after the experimental process and delivered frozen.
Description: Gentaur offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request.
Description: These hepatocytes overcome the functionality, reproducibility, and availability limitations of existing primary cell and tumor-derived cell models by providing the following:Relevant hepatic functionality and phenotypic stabilityExpression of clinical targets that are relevant for liver and metabolic diseaseCompatibility with a wide range of 3D and microphysiological systemsQuality-controlled manufacturing and batch reproducibilityReady supply in a cryopreserved format
Description: Gentaur offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request.
Description: Gentaur's inducible hepatocytes (HP1 Heps-i, single donor, male) can be plated and used in long-term drug induction studies or metabolic studies where long-term incubations are required. Gentaur's cryopreserved primary human hepatocytes are a functional, convenient and economical solution for your drug testing and basic research needs. Each lot is tested for plating and provided with extensive characterization of metabolic functionality to ensure that you receive an ideal model for drug testing, drug development or other metabolic studies. Gentaur's human hepatocytes eliminate the need and expense of obtaining liver tissue, and conducting multiple cell isolations; and Gentaur also provides hepatocyte-tested culture reagents and instructions necessary for thawing and culturing your hepatocytes.Cell Features:Suitable for induction assays or metabolic characterization assays in plated or suspension cultures. Plated cultures are excellent for long-term intrinsic clearance assays.Hepatocytes are cryopreserved ONCE immediately after isolation and purification.Characterization of viability and enzyme activity, including fold-induction of CYP1A2, CYP2B6 and CYP3A4 for HP1 Heps-i.Gentaur guarantees performance and quality.Donor demographics and medical information are available.
Liver Dissociation System 2 (Hepatocytes, Parenchymal hepatocytes), Mouse and Rat
Description: Gentaur's inducible hepatocytes (HP1 Heps-i, single donor, female) can be plated and used in long-term drug induction studies or metabolic studies where long-term incubations are required. Gentaur's cryopreserved primary human hepatocytes are a functional, convenient and economical solution for your drug testing and basic research needs. Each lot is tested for plating and provided with extensive characterization of metabolic functionality to ensure that you receive an ideal model for drug testing, drug development or other metabolic studies. Gentaur's human hepatocytes eliminate the need and expense of obtaining liver tissue, and conducting multiple cell isolations; and Gentaur also provides hepatocyte-tested culture reagents and instructions necessary for thawing and culturing your hepatocytes.Cell Features:Suitable for induction assays or metabolic characterization assays in plated or suspension cultures. Plated cultures are excellent for long-term intrinsic clearance assays.Hepatocytes are cryopreserved ONCE immediately after isolation and purification.Characterization of viability and enzyme activity, including fold-induction of CYP1A2, CYP2B6 and CYP3A4 for HP1 Heps-i.Gentaur guarantees performance and quality.Donor demographics and medical information are available.
Description: Gentaur offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request.
Description: Gentaur offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request.
Description: Gentaur offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request.
Description: Mouse Hepatocyte Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.
Description: Hepatocellular carcinoma (HCC) and normal tissue array, including pathology grade, TNM, stage, HBV and HCV infection information, 30 cases/80 cores, replaced by LV8013b
Normal liver and hepatocellular carcinoma (HCC) tissue array
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
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A scalable 3D culture model was established to study the molecular and cellular biology of human hepatocyte regeneration. Using this model, the important role of Wnt / β-catenin signaling during regeneration of human hepatocytes identified.
