A simple method for isolation and culture of primary hepatocytes from Salvelinus leucomaenis (White-spotted Charr)
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A simple method for isolation and culture of primary hepatocytes from Salvelinus leucomaenis (White-spotted Charr)
White-spotted charr (Salvelinus leucomaenis, S. I.) is adjusted anadromous fish cold water, distributed in the Far East. We have previously reported the complete mitochondrial DNA sequence of white-spotted characters (l. S. imbrius and S. l. Pluvius) in Japan.
In general, fish hepatocytes useful for the study of cellular and biochemical fish. In this study, we isolated hepatocytes from the liver and white speckled charr and use basic methods, such as digestive enzymes and low centrifugation, to analyze the molecular mechanisms involved in specific cellular responses. isolated hepatocytes can be cultured at 5-20 ° C but not 37 ° C. The morphology of hepatocytes has been altered in a manner dependent on the temperature.
The properties of hepatocytes similar to the fish live. In addition, the rate of proliferation and isolated hepatocytes damage depends on the concentration of fetal bovine serum in the culture medium. Taken together, these studies show that this simple method for the isolation and culture of hepatocytes from white speckled charr may be useful for the study of cellular biochemistry and others.
A simple method for isolation and culture of primary hepatocytes from Salvelinus leucomaenis (White-spotted Charr)
A 3D Cell Culture Model Identifies the Wnt / β-Catenin Mediated Inhibition of p53 as Critical Steps for Human Hepatocyte Regeneration
The liver is the organ that is regenerative. While the mature hepatocytes in a state of homeostasis largely silent, after injury, they quickly enter the cell cycle to restore damaged tissue.
In rodents, various models of injury has provided important insights into the fundamentals of molecular regulate hepatocyte proliferation activation silent. However, little is known about the molecular mechanisms of regeneration of human hepatocytes and experimental methods to expand primary human hepatocytes (PHH). Here, the 3D model spheroid of PHH established for the regeneration of hepatocytes and integrative studies of time-lapse multi-omics analysis showed that after the isolation of indigenous people acquire PHH liver regenerative phenotype, as seen in vivo in partial hepatectomy.
However, the proliferation is limited. By analyzing global patterns promoter activity, it is estimated that the activation of Wnt / β-catenin signaling and inhibition of p53 is an important factor that is required for the proliferation of human hepatocytes. functional validation revealed that the activation of Wnt signaling through external cues alone is sufficient to inhibit p53 and proliferation-inducing aging its target PAI1 (SERPINE1) and drive the proliferation of> 50% of all PHH.
Mouse Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)
Description: Hepatocellular carcinoma (HCC) and normal tissue array, including pathology grade, TNM, stage, HBV and HCV infection information, 30 cases/80 cores, replaced by LV8013b
Normal liver and hepatocellular carcinoma (HCC) tissue array
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Hepatocyte Growth Factor (HGF)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Hepatocellular carcinoma (HCC) with adjacent normal liver tissue array
Description: Quantitative sandwich ELISA kit for measuring Human hepatocyte growth factor, HGF in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative sandwich ELISA kit for measuring Human hepatocyte growth factor, HGF in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Human Hepatocyte Growth Factor (HGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Hepatocyte Growth Factor (HGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Hepatocyte growth factor in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Hepatocyte Growth Factor (HGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 1 β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 1 β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 1 β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 6 β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 6 β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 6 β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Recombinant HGF alpha is a non-glycosylated disulfide-linked protein consisting of the alpha chain (463) amino acid residues, and migrates as an approximately 54 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Hepatocyte Growth Factor alpha chain was expressed in E. coli.
Description: Recombinant HGF alpha is a non-glycosylated disulfide-linked protein consisting of the alpha chain (463) amino acid residues, and migrates as an approximately 54 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Hepatocyte Growth Factor alpha chain was expressed in E. coli.
Description: A sandwich ELISA kit for detection of Hepatocyte Growth Factor from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 1 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 1 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 1 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 4 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 4 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 4 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 6 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 6 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Nuclear Factor 6 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in Tissue homogenates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in Tissue homogenates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in Tissue homogenates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in Tissue homogenates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) CLIA Kit
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b)Tissue homogenates and other biological fluids
Human Hepatocyte Growth Factor (HGF) Protein (Active)
Description: A sandwich ELISA for quantitative measurement of Human Hepatocyte growth factor receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Hepatocyte growth factor receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Hepatocyte growth factor receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Hepatocyte nuclear factor 6, ONECUT1 ELISA KIT
Description: A sandwich quantitative ELISA assay kit for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in samples from tissue homogenates or other biological fluids.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in samples from tissue homogenates or other biological fluids.
Human Hepatocyte nuclear factor 1- beta, HNF1B ELISA KIT
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in Tissue homogenates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in Tissue homogenates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in Tissue homogenates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in Tissue homogenates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA Kit
Description: A sandwich ELISA for quantitative measurement of Human Hepatocyte growth factor receptor(MET) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Hepatocyte growth factor receptor(MET) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human Hepatocyte growth factor receptor(MET) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Hepatocyte growth factor receptor(MET) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human Hepatocyte growth factor receptor(MET) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Hepatocyte growth factor receptor, MET ELISA KIT
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Hepatocyte growth factor receptor in samples from serum, plasma, tissue homogenates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Alpha (HNF1A) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Nuclear Factor 1 Alpha (HNF1a) in tissue homogenates, cell lysates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Alpha (HNF1a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Nuclear Factor 1 Alpha (HNF1a) in tissue homogenates, cell lysates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Alpha (HNF1a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Nuclear Factor 1 Alpha (HNF1a) in tissue homogenates, cell lysates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Alpha (HNF1a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hepatocyte Nuclear Factor 1 Alpha (HNF1a) in tissue homogenates, cell lysates and other biological fluids.
Human Hepatocyte Nuclear Factor 1 Alpha (HNF1a) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Hepatocyte Nuclear Factor 1 Alpha (HNF1a) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Human Hepatocyte Nuclear Factor 1 Alpha ELISA Kit (HNF1a)
A scalable 3D culture model was established to study the molecular and cellular biology of human hepatocyte regeneration. Using this model, the important role of Wnt / β-catenin signaling during regeneration of human hepatocytes identified.
