A simple method for isolation and culture of primary hepatocytes from Salvelinus leucomaenis (White-spotted Charr)

White-spotted charr (Salvelinus leucomaenis, S. I.) is adjusted anadromous fish cold water, distributed in the Far East. We have previously reported the complete mitochondrial DNA sequence of white-spotted characters (l. S. imbrius and S. l. Pluvius) in Japan.

In general, fish hepatocytes useful for the study of cellular and biochemical fish. In this study, we isolated hepatocytes from the liver and white speckled charr and use basic methods, such as digestive enzymes and low centrifugation, to analyze the molecular mechanisms involved in specific cellular responses. isolated hepatocytes can be cultured at 5-20 ° C but not 37 ° C. The morphology of hepatocytes has been altered in a manner dependent on the temperature.

The properties of hepatocytes similar to the fish live. In addition, the rate of proliferation and isolated hepatocytes damage depends on the concentration of fetal bovine serum in the culture medium. Taken together, these studies show that this simple method for the isolation and culture of hepatocytes from white speckled charr may be useful for the study of cellular biochemistry and others.

A simple method for isolation and culture of primary hepatocytes from Salvelinus leucomaenis (White-spotted Charr)
A simple method for isolation and culture of primary hepatocytes from Salvelinus leucomaenis (White-spotted Charr)

A 3D Cell Culture Model Identifies the Wnt / β-Catenin Mediated Inhibition of p53 as Critical Steps for Human Hepatocyte Regeneration

The liver is the organ that is regenerative. While the mature hepatocytes in a state of homeostasis largely silent, after injury, they quickly enter the cell cycle to restore damaged tissue.

In rodents, various models of injury has provided important insights into the fundamentals of molecular regulate hepatocyte proliferation activation silent. However, little is known about the molecular mechanisms of regeneration of human hepatocytes and experimental methods to expand primary human hepatocytes (PHH). Here, the 3D model spheroid of PHH established for the regeneration of hepatocytes and integrative studies of time-lapse multi-omics analysis showed that after the isolation of indigenous people acquire PHH liver regenerative phenotype, as seen in vivo in partial hepatectomy.

However, the proliferation is limited. By analyzing global patterns promoter activity, it is estimated that the activation of Wnt / β-catenin signaling and inhibition of p53 is an important factor that is required for the proliferation of human hepatocytes. functional validation revealed that the activation of Wnt signaling through external cues alone is sufficient to inhibit p53 and proliferation-inducing aging its target PAI1 (SERPINE1) and drive the proliferation of> 50% of all PHH.

Human Hepatocytes - Opioid Addicted

HC4230OP 500000
EUR 957

Human Liver PrimaCell: Hepatocytes

2-96121 1 Kit Ask for price

Immortalized Human Hepatocytes - hTERT

T0058 1x106 cells / 1.0 ml Ask for price

Immortalized Human Hepatocytes - Ras

T0059 1x106 cells / 1.0 ml Ask for price

Anti-Hepatocytes antibody

STJ16100176 100 µg
EUR 389

Human Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-47121 1 Set Ask for price

Liver Dissociation System 2 (Hepatocytes, Parenchymal hepatocytes), Mouse and Rat

4-20302 ea Ask for price

Mouse Liver PrimaCell: Hepatocytes

2-82100 1 Kit Ask for price

Rat Liver PrimaCell: Hepatocytes

2-82595 1 Kit Ask for price

Immortalized Monkey Hepatocytes-SV40

T0073 1x106 cells / 1.0 ml Ask for price

Immortalized Rat Hepatocytes - SV40

T0078 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Hepatocytes - SV40

T0101 1x106 cells / 1.0 ml Ask for price

Rat Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-26095 1 Set Ask for price

Mouse Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-33100 1 Set Ask for price

Immortalized Mouse Hepatocytes-Conditionally (ImHep)

T0099 1x106 cells / 1.0 ml Ask for price

Liver Dissociation System 1 (Hepatocytes), Mouse

4-20301 ea Ask for price

Immortalized Hamster Hepatocytes-SV40 T+t

T0071 1x106 cells / 1.0 ml Ask for price

Immortalized Monkey Hepatocytes-SV40 T+t

T0074 1x106 cells / 1.0 ml Ask for price

Liver Dissociation System 4 (Hepatocytes, rat), Rat

4-20304 ea Ask for price

Normal Human Serum

90R-1002 10 ml
EUR 254
Description: Normal Human Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Liver Dissociation System 6 (Hepatocytes,Nonparenchymal), Mouse and Rat

4-20306 ea Ask for price

Normal human exosome sample

P140 - Ask for price

Normal human exosome RNA

P240 - Ask for price

Normal Human Serum (filtered)

90-1013 100 ml
EUR 548
Description: Human Serum Off The Clot from Normal Donors

Liver Dissociation System 3 (Hepatocytes, Kupffer, Parenchymal), Mouse and Rat

4-20303 ea Ask for price

Human Bone PrimaCell: Normal Osteoblasts

2-96012 1 Kit Ask for price

Human Fat PrimaCell2: Normal Preadipocyte

2-96063 1 Kit Ask for price

Human Skin PrimaCell2: Normal Melanocytes

2-96102 1 Kit Ask for price

Human Normal Tissue Blot I

1521 One blot
EUR 453.5
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.

Human Normal Tissue Blot II

1522 One blot
EUR 453.5
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, heart, kidney, liver, lung, pancreas, skeletal muscle, skin, and spleen.

Human Normal Tissue Blot III

1523 One blot
EUR 453.5
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are colon, duodenum, esophagus, ileum, small intestine, jejunum, pancreas, rectum, and stomach.

Human Normal Tissue Blot IV

1524 One blot
EUR 453.5
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are bladder, breast, cervix, kidney, ovary, placenta, prostate, testis, and uterus.

Human Normal Tissue Blot V

1525 One blot
EUR 453.5
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are appendix, kidney, liver, lymph node, peripheral blood leukocyte, spleen, thymus, tonsil, and thyroid.

Human Normal Tissue Blot VI

1526 One blot
EUR 453.5
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.

Normal Goat Serum

9067 50 ml
EUR 100.75
Description: Normal Goat Serum

Normal Bovine Serum

abx098263-100ml 100 ml
EUR 258

Normal Goat Serum

88-B9999G000-S0 10 ml
EUR 133
Description: Control Normal Goat serum

Normal Canine Serum

88-NC25 100 ml
EUR 606
Description: Normal Canine Serum

Normal Horse Serum

88-NE21 100 ml
EUR 30
Description: Control Normal Horse Serum

Normal Goat Serum

88-NG22 500 ml
EUR 30
Description: Control Normal Goat Serum

Normal Goat Serum

88-NG22S 500 ml
EUR 191
Description: Control Normal Goat Serum

Normal Mouse Serum

88-NM35 50 ml
EUR 516
Description: Normal Mouse Serum

Normal Pig Serum

88-NP 500 ml
EUR 243
Description: Normal Pig Serum collected from pigs in the USA

Normal Rabbit Serum

88-NR50 100 ml
EUR 150
Description: Normal Rabbit Serum

Normal Rat Serum

88-NR51 50 ml
EUR 599
Description: Normal Rat Serum

Normal Sheep Serum

88-NS55 100 ml
EUR 126
Description: Normal Sheep Serum

Normal Mouse Serum

88R-1002 5 ml
EUR 203
Description: Normal Mouse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rabbit Serum

88R-1004 10 ml
EUR 206
Description: Normal Rabbit Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Goat Serum

88R-1006 10 ml
EUR 182
Description: Normal Goat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Sheep Serum

88R-1008 10 ml
EUR 182
Description: Normal Sheep Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rat Serum

88R-1010 5 ml
EUR 202
Description: Normal Rat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Chicken Serum

88R-1014 5 ml
EUR 176
Description: Normal Chicken Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Horse Serum

88R-1020 10 ml
EUR 182
Description: Normal Horse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Donkey Serum

88R-1022 10 ml
EUR 182
Description: Normal Donkey Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Pig Serum

88R-1023 2 ml
EUR 116
Description: Normal Pig (Swine) Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Cat Serum

88R-1026 10 ml
EUR 250
Description: Normal Cat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Dog Serum

88R-1028 10 ml
EUR 251
Description: Normal Dog Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Bovine Serum

88R-B001 100 ml
EUR 165
Description: Control Normal Bovine Serum

Normal Donkey Serum

88R-D001 100 ml
EUR 179
Description: Control Normal Donkey Serum

Normal Donkey Serum

88R-D003 10 ml
EUR 149
Description: Control Normal Donkey Serum

Normal Goat Serum

88R-NG001 50 ml
EUR 117
Description: Normal Goat Serum for use as a control or blocking reagent

Normal Pig Serum

88R-P004 1 liter
EUR 673
Description: Control Normal Pig Serum

Normal Sheep Serum

88R-S002 100 ml
EUR 165
Description: Control Normal Sheep Serum

Normal Rabbit Serum

89R-RM001 20 mg
EUR 392
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum

89R-RM002 5 ml
EUR 241
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum

89R-RR001 20 mg
EUR 349
Description: Normal Rabbit Serum (Rat cell adsorbed)

Normal Rabbit Serum

89R-RR002 5 ml
EUR 241
Description: Normal Rabbit Serum (Rat cell adsorbed)

Normal human tissues, 19 organs (2mm)

MNO381 1
EUR 190
Description: Normal tissues from 19 anatomic sites duplicates from 38 individuals, most frequently used for tissue profiling.

Human Bladder PrimaCell2: Normal Bladder Fibroblasts

2-96008 1 Kit Ask for price

Human Brain PrimaCell1: Normal Brain Fibroblasts

2-96013 1 Kit Ask for price

Human Brain PrimaCell7: Normal Meningeal Cells

2-96019 1 Kit Ask for price

Human Brain PrimaCell8: Normal Nerve Astrocytes

2-96020 1 Kit Ask for price

Human Brain PrimaCell9: Normal Nerve Microglia

2-96021 1 Kit Ask for price

Human Brain PrimaCell10: Normal Neuron Cell

2-96022 1 Kit Ask for price

Human Cartilage PrimaCell: Normal Articular Cartilage

2-96043 1 Kit Ask for price

Human Cervix PrimaCell2: Normal Cervical Fibroblasts

2-96045 1 Kit Ask for price

Human Embryo PrimaCell4: Normal Trophoblast Cells

2-96053 1 Kit Ask for price

Human Eye PrimaCell3: Normal Corneal Fibroblasts

2-96059 1 Kit Ask for price

Human Fat PrimaCell1: Normal Adipose Cells

2-96062 1 Kit Ask for price

Human Heart PrimaCell2: Normal Atrial Myocytes

2-96067 1 Kit Ask for price

Human Heart PrimaCell3: Normal Heart Fibroblasts

2-96068 1 Kit Ask for price

Human Heart PrimaCell5: Normal Ventricular Myocytes

2-96070 1 Kit Ask for price

Human Kidney PrimaCell4: Normal Renal Firbroblasts

2-96077 1 Kit Ask for price

Human Kidney PrimaCell6: Normal Renal Podocytes

2-96079 1 Kit Ask for price

Human Lung PrimaCell1: Normal Lung Fibroblasts

2-96081 1 Kit Ask for price

Human Lymph PrimaCell1: Normal Endothelial Cells

2-96087 1 Kit Ask for price

Human Lymph PrimaCell3: Normal Lymphatic Fibroblasts

2-96089 1 Kit Ask for price

Human Mouth PrimaCell: Normal Periodontal Fibroblasts

2-96090 1 Kit Ask for price

Human Ovary PrimaCell2: Normal Ovarian Firbroblasts

2-96095 1 Kit Ask for price

Human Skin PrimaCell1: Normal Epidermal Keratinocytes

2-96101 1 Kit Ask for price

Human Skin PrimaCell: Normal Skin Fibroblasts

2-96103 1 Kit Ask for price

Human Testis PrimaCell: Normal Sertoli Cells

2-96105 1 Kit Ask for price

A scalable 3D culture model was established to study the molecular and cellular biology of human hepatocyte regeneration. Using this model, the important role of Wnt / β-catenin signaling during regeneration of human hepatocytes identified.